The number of kringle IV repeats 3-10 is invariable in the human apo(a) gene

Gene
C HaibachG Utermann

Abstract

The human apolipoprotein(a) (apo(a)) gene is a member of a family of related genes including plasminogen, apo(a)rg-B and apo(a)rg-C, which are clustered on chromosome 6q 2,7. Apo(a) contains ten different types of plasminogen-like kringle IV repeats (K-IV 1-10) one of which (K-IV 2) varies in number resulting in a remarkable size polymorphism of the protein. Sequence analysis of human apo(a) alleles and indirect evidence have suggested that K-IV 1 and K-IV 3-10 are each present once in individual alleles and that the 3' apo(a) region encompassing kringles IV 3-10, kringle V and the protease domain is invariable. To directly test this, we have constructed a restriction map of the apo(a) gene region from genomic DNA and from a yeast artificial chromosome (YAC) (K-IV 13) which contains the entire apo(a) gene. The presence of a 63 kb ClaI fragment encompassing kringles IV 3-10, kringle V and the protease domain and a 46 kb SwaI fragment, spanning kringles IV 5-10, kringle V and the protease domain was demonstrated by PFGE/Southern blotting in 30 unrelated subjects, who represented a range of apo(a) size alleles containing from 11 to 49 kringles. Our analysis demonstrates that the number of kringles IV 3-10 is invariable in the huma...Continue Reading

References

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Citations

May 18, 1999·Current Opinion in Lipidology·G Utermann
May 23, 2001·Protein Science : a Publication of the Protein Society·Q YeZ Jia
Jan 18, 2003·Biological Chemistry·Nigel R CatererHans C Thøgersen
Sep 15, 2007·Medicinski pregled·Biljana Vucković, Mirjana Derić
Apr 15, 2016·Journal of Lipid Research·Konrad SchmidtGerd Utermann
Aug 4, 1999·Current Opinion in Lipidology·H Dieplinger, G Utermann
Oct 12, 2020·Hellenic Journal of Cardiology : HJC = Hellēnikē Kardiologikē Epitheōrēsē·Michael A VavuranakisThorsten M Leucker

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