PMID: 9428698Jan 15, 1998Paper

The NuoI subunit of the Rhodobacter capsulatus respiratory Complex I (equivalent to the bovine TYKY subunit) is required for proper assembly of the membraneous and peripheral domains of the enzyme

European Journal of Biochemistry
M ChevalletJ P Issartel

Abstract

The nuoI gene that encodes a ferredoxin-like subunit of the Rhodobacter capsulatus Complex I (a subunit equivalent to the bovine TYKY subunit) was mutated by homologous recombination. Both a nuoI-deleted mutant (delta nuoI mutant) and a point mutant in which Cys74 was replaced by a serine (C74S mutant) proved to be completely deficient in Complex I activity. These strains were unable to grow under anaerobic photosynthetic conditions. Their cytoplasmic membranes were also characterized by the absence of specific EPR signals assigned to FeS clusters N1 and N2. Immunochemical analysis of the mutant membranes with subunit-specific antibodies showed that the peripheral subunits were not assembled. Trans-complementation of the mutant strains by a native nuoI gene restored the wild-type phenotypes. In the C74S mutant, a limited amount of NuoI subunit still bound to the membraneous domain of Complex I, which is an indication that NuoI directly interacts with this domain. All these results clearly show that NuoI plays a critical role in the connection between the membraneous domain and the peripheral domain of Complex I.

References

Nov 7, 1975·Archives of Microbiology·P F WeaverH Gest
Dec 31, 1982·Biochimica Et Biophysica Acta·H Beinert, S P Albracht
Aug 1, 1993·Journal of Bioenergetics and Biomembranes·V D SledT Ohnishi
Jan 16, 1997·Biochimica Et Biophysica Acta·S P AlbrachtP de Jong
Jan 27, 1997·Molecular & General Genetics : MGG·K Schmidt-BleekL Grohmann

❮ Previous
Next ❯

Citations

Jul 14, 2006·Journal of Inherited Metabolic Disease·Rolf J R J JanssenJan A M Smeitink
Sep 22, 1999·European Journal of Biochemistry·T AlmeidaA Videira
Apr 29, 2006·Journal of Molecular Microbiology and Biotechnology·Dirk FlemmingThorsten Friedrich
Jul 5, 2005·American Journal of Respiratory and Critical Care Medicine·Leigh A Callahan, Gerald S Supinski
Jun 30, 2014·Journal of Bioenergetics and Biomembranes·Motoaki SatoTakao Yagi
Aug 1, 2014·Journal of Bioenergetics and Biomembranes·Eiko Nakamaru-OgisoJoseph A Sakyiama
May 27, 2008·Biochimica Et Biophysica Acta·Michael LazarouMatthew McKenzie
Dec 5, 1998·American Journal of Human Genetics·J LoeffenL van den Heuvel
Sep 8, 2010·Developmental Disabilities Research Reviews·Shane L ReaMarni J Falk
Sep 17, 2003·The Journal of Biological Chemistry·Dirk FlemmingThorsten Friedrich
May 9, 2001·The Journal of Biological Chemistry·N Kashani-PoorU Brandt
Apr 17, 2002·Biochimica Et Biophysica Acta·Pierre LescuyerJoël Lunardi
Jun 19, 1998·Biochimica Et Biophysica Acta·A DupuisJ P Issartel

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.