The obligate intracellular parasite Toxoplasma gondii secretes a soluble phosphatidylserine decarboxylase.

The Journal of Biological Chemistry
Nishith GuptaD R Voelker

Abstract

Toxoplasma gondii is an obligate intracellular parasite capable of causing fatal infections in immunocompromised individuals and neonates. Examination of the phosphatidylserine (PtdSer) metabolism of T. gondii reveals that the parasite secretes a soluble form of PtdSer decarboxylase (TgPSD1), which preferentially decarboxylates liposomal PtdSer with an apparent K(m) of 67 μM. The specific enzyme activity increases by 3-fold during the replication of T. gondii, and soluble phosphatidylserine decarboxylase (PSD) accounts for ∼20% of the total PSD, prior to the parasite egress from the host cells. Extracellular T. gondii secreted ∼20% of its total PSD activity at 37 °C, and the intracellular Ca(2+) chelator 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid tetrakis (acetoxymethyl ester) inhibited the process by 50%. Cycloheximide, brefeldin A, ionic composition of the medium, and exogenous PtdSer did not modulate the enzyme secretion, which suggests a constitutive discharge of a presynthesized pool of PSD in axenic T. gondii. TgPSD1 consists of 968 amino acids with a 26-amino acid hydrophobic peptide at the N terminus and no predicted membrane domains. Parasites overexpressing TgPSD1-HA secreted 10-fold more activity compar...Continue Reading

References

Dec 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·R G Donald, D S Roos
Nov 25, 1997·Biochimica Et Biophysica Acta·O Kuge, M Nishijima
Nov 25, 1997·Biochimica Et Biophysica Acta·D R Voelker
Aug 1, 1959·Canadian Journal of Biochemistry and Physiology·E G BLIGH, W J DYER
Mar 9, 2004·International Journal for Parasitology·Kami Kim, Louis M Weiss
Mar 31, 2004·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Jean E Vance, Dennis E Vance
Jun 28, 2005·International Journal for Parasitology·Corinne MercierMarie-France-Cesbron Delauw
Jul 9, 2005·The Journal of Biological Chemistry·Xing W ZhouVern B Carruthers
Jan 25, 2008·Nature Reviews. Molecular Cell Biology·Gerrit van MeerGerald W Feigenson
Mar 5, 2008·Traffic·Marie-France Cesbron-DelauwCorinne Mercier
Aug 2, 2008·Microbes and Infection·Kami Kim, Louis M Weiss
Jan 24, 2009·IUBMB Life·Irmgard Schuiki, Günther Daum
Jul 21, 2009·Proceedings of the National Academy of Sciences of the United States of America·Martin BlumeNishith Gupta

❮ Previous
Next ❯

Citations

Sep 27, 2012·Biochimica Et Biophysica Acta·Luce Farine, Peter Bütikofer
Sep 11, 2012·Biochimica Et Biophysica Acta·Jean E Vance, Guergana Tasseva
Jul 6, 2013·Progress in Lipid Research·Srinivasan RamakrishnanPeter Bütikofer
Dec 24, 2015·The Journal of Biological Chemistry·Viviana PszennyIsabelle Coppens
Dec 21, 2019·Advanced Pharmaceutical Bulletin·Esmaeil AbasiHabib Mohammadzadeh Hajipirloo
Dec 17, 2017·The Journal of Biological Chemistry·Jae-Yeon ChoiDennis R Voelker
Jan 25, 2021·The Journal of Biological Chemistry·Kai ChenNishith Gupta
Jan 1, 2021·Frontiers in Cellular and Infection Microbiology·Bruno Martorelli Di Genova, Laura J Knoll

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

Autoimmune Polyendocrinopathies

Autoimmune polyendocrinopathies, also called polyglandular autoimmune syndromes (PGASs), or polyendocrine autoimmune syndromes(PASs), are a heterogeneous group of rare diseases characterized by autoimmune activity against more than one endocrine organ, although non-endocrine organs can be affected. Discover the latest research on autoimmune polyendocrinopathies here.