The obstruction factor in size-exclusion chromatography. 2. The interparticle, intraparticle, and total obstruction factors

Journal of Chromatography. a
Dustin J Richard, André M Striegel

Abstract

"Obstruction factor" is a generic rubric under which are usually gathered the interparticle, intraparticle, stationary phase, and total obstruction factors, γ(e), γ(p), γ(s), and γ(t), respectively. These, in turn, affect longitudinal diffusion and stationary, mobile phase, and stagnant mobile phase mass transfer. We conclude here our investigation into the various obstruction factors operative in size-exclusion chromatography (SEC). Stop-flow experiments were employed to determine either the interparticle (for analytes with K(SEC)=0) or the total (for analytes with K(SEC)>0) obstruction factor, and these results were combined with those from variable-flow-rate experiments which provided the intraparticle obstruction factor. Because of minimal enthalpic interactions between the analytes and stationary phase, in SEC γ(s)≈0, which allows for isolation of the other obstruction factors. A relationship between γ(t), γ(e), and γ(p) was proposed for SEC, based on previous independent work and dependent upon the various column porosities. This relationship was extended to hydrodynamic chromatography, a technique in which, ideally, both γ(s) and γ(p) are equal to zero.

References

Apr 20, 2004·Journal of Chromatography. a·André M Striegel
Sep 3, 2008·Analytical and Bioanalytical Chemistry·Amandaa K Brewer, André M Striegel
Apr 7, 2009·Journal of Chromatography. a·Kanji MiyabeGeorges Guiochon

❮ Previous
Next ❯

Citations

Aug 3, 2014·Journal of Chromatography. a·André M Striegel
Mar 19, 2013·The Journal of Physical Chemistry. a·Benjamin J Rogers, Mary J Wirth

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.