The OC43 human coronavirus envelope protein is critical for infectious virus production and propagation in neuronal cells and is a determinant of neurovirulence and CNS pathology

Virology
Jenny K StodolaPierre J Talbot

Abstract

The OC43 strain of human coronavirus (HCoV-OC43) is an ubiquitous respiratory tract pathogen possessing neurotropic capacities. Coronavirus structural envelope (E) protein possesses specific motifs involved in protein-protein interaction or in homo-oligomeric ion channel formation, which are known to play various roles including in virion morphology/assembly and in cell response to infection and/or virulence. Making use of recombinant viruses either devoid of the E protein or harboring mutations either in putative transmembrane domain or PDZ-binding motif, we demonstrated that a fully functional HCoV-OC43 E protein is first needed for optimal production of recombinant infectious viruses. Furthermore, HCoV-OC43 infection of human epithelial and neuronal cell lines, of mixed murine primary cultures from the central nervous system and of mouse central nervous system showed that the E protein is critical for efficient and optimal virus replication and propagation, and thereby for neurovirulence.

Citations

May 18, 2020·Journal of Neurovirology·Ali SepehrinezhadSajad Sahab Negah
Jul 21, 2020·Reviews in Medical Virology·Osama S Abdelaziz, Zuraiha Waffa
Sep 26, 2020·Frontiers in Neurology·Gilmara Gomes de AssisEugene V Gasanov
Sep 19, 2020·International Journal of Molecular Sciences·Elisabetta MeacciFederica Pierucci
Sep 15, 2020·Journal of Alzheimer's Disease : JAD·Vicky YamamotoBabak Kateb
Oct 10, 2020·Life Sciences·Sairaj Satarker, Madhavan Nampoothiri
Dec 29, 2020·Frontiers in Immunology·Gisela Canedo-MarroquínAlexis M Kalergis
Feb 3, 2021·International Journal of Molecular Sciences·Nicholas A Wong, Milton H Saier
Apr 8, 2021·Acta Crystallographica. Section D, Structural Biology·Francisco J Barrantes

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Methods Mentioned

BETA
transfection
profiler
Assay
PCR
protein assay

Software Mentioned

cell profiler
Procapture system
CellProfiler
StepOne

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