PMID: 9182251Feb 1, 1996Paper

The occurrence of protein kinase C theta and lambda isoforms in retina of different species

Neurochemical Research
R J McCordN N Osborne

Abstract

The localization and immunochemical identification of the novel protein kinase C theta (nPKC theta) and the atypical protein kinase C lambda (aPKC lambda) isoforms in retinas of different species were analyzed by immunohistochemistry and SDS-PAGE/Western blotting. nPKC theta immunoreactivity is associated with bipolar cells of mammalian (rabbit, rat and guinea pig) retinas but not the non-mammalian goldfish retina which has a lower concentration of nPKC theta. However, SDS-PAGE and Western blotting data indicate the antigen recognized by the nPKC theta monoclonal antibody in the retina is of a lower molecular weight than that expected for nPKC theta. This would suggest nPKC theta is more susceptible to degradation/breakdown than other PKC isoforms found in the retina or that the nPKC theta antibody may be recognizing an unknown retinal antigen. A comparison of nPKC theta and cPKC alpha immunoreactivities in bipolar cells shows unique distributions exist for the two isoforms. nPKC theta is present in the developing retina at an earlier stage than cPKC alpha. The typical 'transport' of cPKC alpha toward axonal terminals by phorbol-12,13-dibutyrate does not occur for nPKC theta yet both are translocated from the cytosolic to membr...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Aug 1, 1994·Cellular Signalling·I P UdovichenkoD J Takemoto
Oct 1, 1994·European Journal of Biochemistry·G BaierA Altman
Apr 15, 1993·The Biochemical Journal·H Hug, T F Sarre
May 1, 1993·Neurochemistry International·K P Huang, F L Huang
Feb 1, 1997·Neurochemistry International·J P WoodN N Osborne

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Citations

Feb 1, 1997·Neurochemistry International·J P WoodN N Osborne
Feb 8, 2006·The Journal of Veterinary Medical Science·Taekyun ShinHeechul Kim

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