The Oct4 and Nanog transcription network regulates pluripotency in mouse embryonic stem cells

Nature Genetics
Yuin-Han LohHuck-Hui Ng

Abstract

Oct4 and Nanog are transcription factors required to maintain the pluripotency and self-renewal of embryonic stem (ES) cells. Using the chromatin immunoprecipitation paired-end ditags method, we mapped the binding sites of these factors in the mouse ES cell genome. We identified 1,083 and 3,006 high-confidence binding sites for Oct4 and Nanog, respectively. Comparative location analyses indicated that Oct4 and Nanog overlap substantially in their targets, and they are bound to genes in different configurations. Using de novo motif discovery algorithms, we defined the cis-acting elements mediating their respective binding to genomic sites. By integrating RNA interference-mediated depletion of Oct4 and Nanog with microarray expression profiling, we demonstrated that these factors can activate or suppress transcription. We further showed that common core downstream targets are important to keep ES cells from differentiating. The emerging picture is one in which Oct4 and Nanog control a cascade of pathways that are intricately connected to govern pluripotency, self-renewal, genome surveillance and cell fate determination.

References

Mar 29, 1990·Nature·H R SchölerP Gruss
Jun 1, 1990·Current Opinion in Cell Biology·M AffolterW J Gehring
Jul 21, 2001·Stem Cells·M Pesce, H R Schöler
Jul 27, 2001·Bioinformatics·G PavesiG Pesole
Nov 1, 2001·Annual Review of Cell and Developmental Biology·A G Smith
Nov 2, 2001·Nature·P J Donovan, J Gearhart
Mar 14, 2002·Proceedings of the National Academy of Sciences of the United States of America·Ying GuoRobert Hromas
Oct 17, 2002·Genes & Development·Lynn A HannaPatricia A Labosky
Nov 28, 2002·Nature Genetics·Jonathan R Pollack, Vishwanath R Iyer
Jan 7, 2003·Genes & Development·Ariel A AvilionRobin Lovell-Badge
Apr 1, 2003·Molecular and Cellular Biology·Yoshimi TokuzawaShinya Yamanaka
Oct 7, 2003·Proceedings of the National Academy of Sciences of the United States of America·Rebecca MartoneMichael Snyder
Nov 19, 2003·Developmental Biology·David A F LoebelPatrick P L Tam
Mar 3, 2004·Molecular and Cellular Biology·Jonathan E DodgeEn Li
Mar 26, 2004·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Ian R Adams, Anne McLaren
Aug 12, 2004·BMC Developmental Biology·Ralph BrandenbergerMahendra Rao
Sep 21, 2004·Oncogene·Ian Chambers, Austin Smith
Nov 24, 2004·The Journal of Biological Chemistry·Sayaka Okumura-NakanishiFuyuki Ishikawa
Dec 8, 2004·The Journal of Cell Biology·Lifeng Xu, Elizabeth H Blackburn
Dec 21, 2004·Nucleic Acids Research·Huaiyu MiPaul D Thomas
Jan 28, 2005·Development·Peter CartwrightStephen Dalton
Mar 4, 2005·Molecular and Cellular Biology·Takao KurodaTakashi Tada
Mar 12, 2005·Nucleic Acids Research·Thomas A Down, Tim J P Hubbard
Apr 30, 2005·The Journal of Biological Chemistry·David J RoddaPaul Robson
Jul 1, 2005·Nature·Tae Hoon KimBing Ren
Sep 13, 2005·Cell·Laurie A BoyerRichard A Young

❮ Previous
Next ❯

Citations

Sep 13, 2007·Stem Cell Reviews·Andrew C BoquestPhilippe Collas
Jun 6, 2008·Cellular and Molecular Life Sciences : CMLS·L A Davis, N I Zur Nieden
Aug 8, 2009·Cellular and Molecular Life Sciences : CMLS·Manal BosnaliFrank Edenhofer
Nov 28, 2012·Development Genes and Evolution·David A Gold, David K Jacobs
Nov 8, 2007·Cell and Tissue Research·Steven Hatfield, Hannele Ruohola-Baker
Jan 31, 2012·Cell and Tissue Research·Eizo NakagawaHan-Sung Jung
Jan 24, 2012·Bulletin of Experimental Biology and Medicine·E V Grigor'evaS M Zakian
Jul 25, 2007·Cancer Metastasis Reviews·Bryan C Barnhart, M Celeste Simon
Sep 22, 2010·Molecular Biology Reports·Haiying LiuYongmei Guan
Sep 20, 2007·Science in China. Series C, Life Sciences·YuXiao LiuXueTao Pei
Feb 22, 2011·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Aiping FangHong Hu
Jun 19, 2013·In Vitro Cellular & Developmental Biology. Animal·Shahram DarabiTaher Taheri
Jan 16, 2010·Molecular Biotechnology·Philippe Collas
May 23, 2012·Molecular Neurobiology·Aisha Iman AbdullahTao Sun
Aug 7, 2013·Seminars in Cell & Developmental Biology·Kevin Andrew Uy Gonzales, Huck-Hui Ng
Jan 24, 2009·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Rui Zhang, Bing Su
Jan 16, 2010·Cell Death and Differentiation·S Mora-CastillaF J Bedoya
Mar 11, 2011·Cell Death & Disease·E M Abdelalim, I Tooyama
Jul 1, 2009·Cell Research·Lingyi ChenGeorge Q Daley
Jul 16, 2010·Cell Research·Lingyi ChenGeorge Q Daley
Feb 16, 2011·Cell Research·Bernadett Papp, Kathrin Plath
Dec 12, 2012·Cell Research·Jia-Chi Yeo, Huck-Hui Ng
Feb 9, 2012·Molecular Therapy : the Journal of the American Society of Gene Therapy·Robert StraussJiri Bartek
May 15, 2013·Molecular Therapy : the Journal of the American Society of Gene Therapy·Nao SuzukiHiromitsu Nakauchi
Dec 22, 2007·Nature·Ian ChambersAustin Smith
Mar 26, 2008·Nature·Sanjay K SinghSadhan Majumder
Aug 20, 2010·Nature·Michael H KageyRichard A Young
Nov 10, 2006·Nature Biotechnology·Anthony C F Perry
May 5, 2011·Nature Cell Biology·Huck-Hui Ng, M Azim Surani
Oct 3, 2006·Nature Cell Biology·Mélanie Bilodeau, Guy Sauvageau
Feb 12, 2008·Nature Cell Biology·Jianming JiangHuck-Hui Ng

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Fate Specification

Cell fate specification is determined by complex signal transduction pathways including Notch and the Sonic hedgehog signaling pathway. Here is the latest research on regulation of cell fate determination.