PMID: 2505259Sep 1, 1989Paper

The octamer motif is a B-lymphocyte-specific regulatory element of the HLA-DR alpha gene promoter

Proceedings of the National Academy of Sciences of the United States of America
P A ShermanJ P Ting

Abstract

The human class II gene, HLA-DR alpha, contains an octanucleotide sequence ATTTGCAT located approximately 40 base pairs upstream of the transcription initiation site. We have investigated the transcriptional function of the DR alpha octamer in human B-lymphoblastoid cells and non-B cells. Deletion and substitution mutagenesis of the octamer sequence greatly reduced the activity of the DR alpha promoter in both in vivo and in vitro cell-free transcription systems of B-cell origin. Conversely, these mutations did not affect promoter activity in several non-B-cell lines that express the DR alpha gene. Removal of octamer-binding proteins by in vivo titration with an octamer-containing competitor plasmid reduced DR alpha promoter activity in B-lymphoblastoid cells. These results suggest that a protein-octamer interaction, most likely involving the B-cell-specific octamer binding protein (OTF-2), is required for DR alpha promoter function in B-lymphoblastoid cells but not in non-B cells.

References

Nov 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·S Y TsangB M Peterlin
Jun 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·P A ShermanJ P Ting
Jun 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·H K DasS M Weissman
Sep 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·H SaitoS Tonegawa
Nov 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·Y BergmanD Baltimore
Dec 1, 1982·The Journal of Experimental Medicine·A N HoughtonL J Old

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Citations

Jan 1, 1993·Immunologic Research·J P Ting
Oct 18, 1994·Biochemical Pharmacology·A CiucciP Giacomini
Mar 1, 1996·Molecular Immunology·G BannishJ S Lee
Feb 1, 1991·Current Opinion in Immunology·C J Kara, L H Glimcher
Feb 1, 1993·Current Opinion in Immunology·J P Ting, A S Baldwin
Feb 1, 1997·Current Opinion in Immunology·J M Boss
Oct 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J P CogswellJ P Ting
Aug 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·K L Wright, J P Ting
Apr 1, 1995·The Journal of Experimental Medicine·K L WrightJ P Ting
Aug 15, 1996·Genes & Development·K L CepekP A Sharp

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