The oligomer plasticity of the small heat-shock protein Lo18 from Oenococcus oeni influences its role in both membrane stabilization and protein protection

The Biochemical Journal
Magali MaitreJean Guzzo

Abstract

The ability of the small Hsp (heat-shock protein) Lo18 from Oenococcus oeni to modulate the membrane fluidity of liposomes or to reduce the thermal aggregation of proteins was studied as a function of the pH in the range 5-9. We have determined by size-exclusion chromatography and analytical ultracentrifugation that Lo18 assembles essentially as a 16-mer at acidic pH. Its quaternary structure evolves to a mixture of lower molecular mass oligomers probably in dynamic equilibrium when the pH increases. The best Lo18 activities are observed at pH 7 when the particle distribution contains a major proportion of dodecamers. At basic pH, particles corresponding to a dimer prevail and are thought to be the building blocks leading to oligomerization of Lo18. At acidic pH, the dimers are organized in a double-ring of stacked octamers to form the 16-mer as shown by the low-resolution structure determined by electron microscopy. Experiments performed with a modified protein (A123S) shown to preferentially form dimers confirm these results. The α-crystallin domain of Methanococcus jannaschii Hsp16.5, taken as a model of the Lo18 counterpart, fits with the electron microscopy envelope of Lo18.

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Citations

Jun 19, 2012·The International Journal of Biochemistry & Cell Biology·Jean Guzzo
Dec 10, 2013·Protein Science : a Publication of the Protein Society·Xinmiao FuChao Wang
Jun 20, 2016·International Journal of Food Microbiology·Stéphanie WeidmannJean Guzzo
Mar 31, 2017·Microbiology·Cosette Grandvalet
Mar 19, 2019·Critical Reviews in Food Science and Nutrition·Daniela FioccoVittorio Capozzi
Feb 14, 2019·Cell Stress & Chaperones·Serena CarraRobert M Tanguay
Apr 18, 2020·Cell Stress & Chaperones·Igor Obuchowski, Krzysztof Liberek
Dec 25, 2019·Archives of Biochemistry and Biophysics·Alejandra A CovarrubiasDavid F Rendón-Luna
Jun 1, 2021·Frontiers in Molecular Biosciences·Igor ObuchowskiKrzysztof Liberek
Oct 7, 2021·Microbiology Spectrum·Cristobal A OnettoSimon A Schmidt

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