The omptins of Yersinia pestis and Salmonella enterica cleave the reactive center loop of plasminogen activator inhibitor 1.

Journal of Bacteriology
Johanna HaikoTimo K Korhonen

Abstract

Plasminogen activator inhibitor 1 (PAI-1) is a serine protease inhibitor (serpin) and a key molecule that regulates fibrinolysis by inactivating human plasminogen activators. Here we show that two important human pathogens, the plague bacterium Yersinia pestis and the enteropathogen Salmonella enterica serovar Typhimurium, inactivate PAI-1 by cleaving the R346-M347 bait peptide bond in the reactive center loop. No cleavage of PAI-1 was detected with Yersinia pseudotuberculosis, an oral/fecal pathogen from which Y. pestis has evolved, or with Escherichia coli. The cleavage and inactivation of PAI-1 were mediated by the outer membrane proteases plasminogen activator Pla of Y. pestis and PgtE protease of S. enterica, which belong to the omptin family of transmembrane endopeptidases identified in Gram-negative bacteria. Cleavage of PAI-1 was also detected with the omptins Epo of Erwinia pyrifoliae and Kop of Klebsiella pneumoniae, which both belong to the same omptin subfamily as Pla and PgtE, whereas no cleavage of PAI-1 was detected with omptins of Shigella flexneri or E. coli or the Yersinia chromosomal omptins, which belong to other omptin subfamilies. The results reveal a novel serpinolytic mechanism by which enterobacterial s...Continue Reading

References

Nov 6, 1992·Science·O A SodeindeJ D Goguen
Jan 1, 1991·Annual Review of Cell Biology·K T Preissner
Oct 1, 1988·Infection and Immunity·O A Sodeinde, J D Goguen
Mar 1, 1982·Infection and Immunity·P J SansonettiS B Formal
Mar 1, 1984·Infection and Immunity·T Une, R R Brubaker
Dec 5, 1993·Journal of Molecular Biology·A Sali, T L Blundell
Jan 1, 1994·Journal of Bacteriology·A KaufmannU Henning
Feb 1, 1996·Journal of Molecular Graphics·W HumphreyK Schulten
Nov 26, 1999·Proceedings of the National Academy of Sciences of the United States of America·M AchtmanE Carniel
Feb 13, 2001·Annual Review of Medicine·M E Ohl, S I Miller
Feb 24, 2001·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·T KanekoS Tabata
Jul 27, 2002·Journal of Bacteriology·Wen DengRobert D Perry
Nov 27, 2002·Applied and Environmental Microbiology·Gayle C McGheeAlan L Jones
Mar 12, 2003·The Journal of Experimental Medicine·Lawrence L JohnsonStephen T Smiley
Sep 11, 2004·Proceedings of the National Academy of Sciences of the United States of America·P S G ChainE Garcia
Dec 4, 2004·Biological Chemistry·Koki Matsumoto
Jan 7, 2005·Journal of Thrombosis and Haemostasis : JTH·H R Lijnen
Feb 1, 2005·Trends in Microbiology·Kaarina LähteenmäkiTimo K Korhonen
Feb 11, 2005·Proceedings of the National Academy of Sciences of the United States of America·E G RubyE P Greenberg
Mar 12, 2005·Cellular Microbiology·Kaarina LähteenmäkiTimo K Korhonen
Dec 31, 2005·Nucleic Acids Research·Neil D RawlingsAlan J Barrett
Mar 29, 2006·Proceedings of the National Academy of Sciences of the United States of America·Florent SebbaneB Joseph Hinnebusch

❮ Previous
Next ❯

Citations

Feb 12, 2011·BMC Evolutionary Biology·Johanna HaikoTimo K Korhonen
Oct 21, 2015·Critical Reviews in Microbiology·Marijke PeetermansPeter Verhamme
Jul 17, 2012·Immunobiology·Manuel T Silva, Nazaré T Silva Pestana
Sep 18, 2015·Microbiology·Sara Schesser BartraGregory V Plano
May 25, 2013·Molecular Microbiology·Matthew B LawrenzVirginia L Miller
Aug 14, 2010·Journal of Thrombosis and Haemostasis : JTH·M Valls SerónJ C M Meijers
Jul 8, 2015·Journal of Thrombosis and Haemostasis : JTH·T K Korhonen
May 13, 2014·FEMS Microbiology Letters·Veena PremjaniJohn A Samis
Aug 30, 2016·Biochimica Et Biophysica Acta. General Subjects·Amit RahiRakesh Bhatnagar
Apr 1, 2015·Infection and Immunity·John R BrannonHervé Le Moual
Nov 11, 2015·Infection and Immunity·Daniel L ZimblerWyndham W Lathem
Oct 7, 2015·Infection and Immunity·Justin L EddyWyndham W Lathem
Sep 10, 2015·Journal of Bacteriology·John R BrannonHervé Le Moual
Nov 1, 2018·Current Medicinal Chemistry·Bożena Futoma-KołochAndrzej Gamian
Mar 15, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Deyan LuoStephen T Smiley
Mar 15, 2020·FEBS Letters·Eric S Krukonis, Joshua J Thomson
Mar 5, 2020·The Journal of Infectious Diseases·Srijon K BanerjeeRoger D Pechous
Nov 19, 2020·Biomolecules·Florent SebbaneAndrey P Anisimov
Feb 25, 2021·Emerging Infectious Diseases·Federica GigliucciValeria Michelacci
Jul 9, 2021·International Journal of Medical Microbiology : IJMM·Paweł SerekIwona Bednarz-Misa

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.