The on-off action of Forkhead protein O3a in endotoxin tolerance of Kupffer cells depends on the PI3K/AKT pathway

International Immunopharmacology
Di MuWenfeng Zhang

Abstract

The endotoxin tolerance (ET) of Kupffer cells (KCs) is an important protective mechanism for limiting endotoxin shock. As a key anti-inflammatory molecule, the roles and mechanism of Forkhead protein O3a (Foxo3a) in ET of KCs are not yet well understood. ET and nonendotoxin tolerance (NET) KCs models were established in vitro and in vivo. The levels of cytokines were detected by enzyme-linked immunosorbent assay (ELISA). The protein expression and phosphorylation levels were detected by western blotting (WB). Changes in the localization of nuclear factor kappa B (NF-κB) and Foxo3a in KCs were detected by immunofluorescence assays. KCs apoptosis and survival rates were detected by flow cytometry and an automatic cell counter, respectively. The activity of NF-κB and the levels of p-Foxo3a and tumor necrosis factor (TNF-α) in the ET group were significantly lower than those in the NET group, while the levels of Foxo3a and interleukin 10 (IL-10) in the ET group were significantly higher than those in the NET group. Overexpression of Foxo3a or the use of a phosphatidylinositol-3-hydroxykinase (PI3K) inhibitor suppressed the activation of NF-κB by decreasing the levels of p-Foxo3a by inhibiting the activity of PI3K/AKT, which improve...Continue Reading

References

Sep 29, 2009·Trends in Immunology·Subhra K Biswas, Eduardo Lopez-Collazo
Dec 18, 2013·Immunology Letters·Pei-zhi LiKun He
Jul 16, 2014·Nature Communications·Lin LuoJennifer L Stow
Jan 22, 2015·Science Signaling·Arnaud DeladeriereLen R Stephens
Nov 13, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Matthew G ThompsonStephanie K Watkins
Nov 1, 2016·Nature·Megan M KanedaJudith A Varner
Mar 18, 2017·Molecular Immunology·Peizhi LiJianping Gong
Sep 15, 2017·Journal of Physiology and Biochemistry·Wenfeng ZhangXiaoling Wu
Mar 7, 2018·Nature Reviews. Clinical Oncology·Filip JankuFunda Meric-Bernstam
Jul 12, 2018·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Yuping ZhouShukun Fu
Sep 25, 2018·Journal of Cellular Biochemistry·Dan LiuYuxia Xiong
Dec 5, 2018·Scientific Reports·Wilson Mitsuo Tatagiba KuwabaraTatiana Carolina Alba-Loureiro
Jun 14, 2019·Oncotarget·Mariana M C AndradeFrancisco G Soriano

❮ Previous
Next ❯

Citations

May 29, 2020·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Chao YanKui-Yang Zheng

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Related Papers

Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology
Sheng-wei LiJianping Gong
Hepatology : Official Journal of the American Association for the Study of Liver Diseases
Qiang YouCynthia Ju
© 2021 Meta ULC. All rights reserved