The Oncogenic Activity of miR-29b-1-5p Induces the Epithelial-Mesenchymal Transition in Oral Squamous Cell Carcinoma

Journal of Clinical Medicine
Miyako Kurihara-ShimomuraTadaaki Kirita

Abstract

The relationship between miR-29b-1-5p and c-Met proto-oncogene in oral squamous cell carcinoma (OSCC) remains to be investigated. This study aimed to reveal the role of miR-29b-1-5p in the pathogenesis of OSCC using molecular and biological analyses. We investigated the expression of miR-29b-1-5p, c-Met, and markers of the epithelial-mesenchymal transition (EMT) in the tissues of 49 patients with OSCC and in human OSCC cells with different tumorigenicity. Further, we determined the effects of miR-29b-1-5p on the phenotypes of OSCC cell lines. The expression levels of miR-29b-1-5p in most patients with OSCC were higher than those of the normal oral epithelium. In OSCC, upregulation of miR-29b-1-5p significantly correlated with histological grade, the EMT, and the immunohistochemical grade, indicated by c-Met expression. The prognosis was poor for patients with miR-29b-1-5p expression and coexpression of miR-29b-1-5p and c-Met. In OSCC cells exhibiting the EMT phenotype, knockdown of miR-29b-1-5p suppressed the EMT, which was recovered by enforced expression of c-Met. Further, the mRNA encoding cadherin 1 (CDH1) was a direct target of miR-29b-1-5p. Our results suggest that miR-29b-1-5p acts as an oncogenic miRNA that synergizes w...Continue Reading

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Citations

Mar 29, 2020·Frontiers in Cell and Developmental Biology·Hong ZhanJun Lin
Apr 4, 2021·International Journal of Molecular Sciences·Kumar RishabhAjaikumar B Kunnumakkara
Apr 10, 2021·BioMed Research International·Luigi LainoMarco Cicciù
Oct 8, 2021·Cell Biology and Toxicology·Boyuan Zhao, Yonggang Lv

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Methods Mentioned

BETA
laser
Reverse-Transcription
PCR

Software Mentioned

JMP13
mivroRNA

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