The One-carbon Carrier Methylofuran from Methylobacterium extorquens AM1 Contains a Large Number of α- and γ-Linked Glutamic Acid Residues.

The Journal of Biological Chemistry
Jethro L HemmannJulia A Vorholt

Abstract

Methylobacterium extorquens AM1 uses dedicated cofactors for one-carbon unit conversion. Based on the sequence identities of enzymes and activity determinations, a methanofuran analog was proposed to be involved in formaldehyde oxidation in Alphaproteobacteria. Here, we report the structure of the cofactor, which we termed methylofuran. Using an in vitro enzyme assay and LC-MS, methylofuran was identified in cell extracts and further purified. From the exact mass and MS-MS fragmentation pattern, the structure of the cofactor was determined to consist of a polyglutamic acid side chain linked to a core structure similar to the one present in archaeal methanofuran variants. NMR analyses showed that the core structure contains a furan ring. However, instead of the tyramine moiety that is present in methanofuran cofactors, a tyrosine residue is present in methylofuran, which was further confirmed by MS through the incorporation of a (13)C-labeled precursor. Methylofuran was present as a mixture of different species with varying numbers of glutamic acid residues in the side chain ranging from 12 to 24. Notably, the glutamic acid residues were not solely γ-linked, as is the case for all known methanofurans, but were identified by NMR ...Continue Reading

References

Jan 1, 1990·Annual Review of Biochemistry·A A DiMarcoR S Wolfe
May 1, 1987·Archives of Biochemistry and Biophysics·T A BobikR S Wolfe
Feb 15, 1983·European Journal of Biochemistry·J T KeltjensG D Vogels
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Mar 27, 2001·Methods in Enzymology·S Shima, R K Thauer
Sep 5, 2001·European Journal of Biochemistry·B K Pomper, J A Vorholt
Sep 5, 2002·Archives of Microbiology·Julia A Vorholt
Mar 12, 2004·Molecular Biology and Evolution·Ludmila ChistoserdovaMazy E Lidstrom
Feb 8, 2005·Genome Biology·Ludmila ChistoserdovaMary E Lidstrom
Feb 18, 2005·Biochimica Et Biophysica Acta·Nicole D KezmarskyRobert H White
Dec 21, 2010·Analytical Chemistry·Patrick KieferJulia A Vorholt
Jul 1, 2014·Biochemistry·Danielle MillerRobert H White

❮ Previous
Next ❯

Citations

Jul 22, 2020·Annual Review of Microbiology·Seigo ShimaUlrich Ermler
Nov 30, 2019·Proceedings of the National Academy of Sciences of the United States of America·Jethro L HemmannJulia A Vorholt

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Protein Structures

Bacterial protein structures can expedite the development of novel antibiotics. Here is the latest research on bacterial proteins and the resolution of their structures.

Bacterial Protein Structures (ASM)

Bacterial protein structures can expedite the development of novel antibiotics. Here is the latest research on bacterial proteins and the resolution of their structures.

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.