The ontogeny of specific retrograde transport of nerve growth factor by motoneurons of the brainstem and spinal cord

Developmental Biology
D B Wayne, M B Heaton

Abstract

Radiolabeled Nerve Growth Factor (NGF) was injected into either the mandibular process of the first visceral arch or the limb bud of chick embryos at Days 3.5-14 or Days 4-13 of incubation, respectively. Control embryos received injections of labeled cytochrome-C or labeled NGF plus an excess of unlabeled NGF. The tissues were then processed for autoradiography. The 125I-NGF was retrogradely transported by motoneurons of the trigeminal (V) motor nucleus on Days 3.5-8 of incubation, but not at later stages. Similar transport was seen in motoneurons of the spinal cord lateral motor column from Days 4-10 of incubation, but not at later stages. Sensory neurons of the V ganglion and of the dorsal root ganglia transported NGF at all injection ages. In no instance was the 125I-cytochrome-C transported by sensory or motor neurons. The injection of an excess of cold NGF along with labeled NGF resulted in no evidence of retrograde transport of the labeled NGF indicating that the transport was saturable. The time of transport by these brainstem and spinal cord motoneurons corresponds closely to the points during development at which they have been found to exhibit specific NGF binding. The present results, then, provide further evidence f...Continue Reading

References

Dec 3, 1999·The European Journal of Neuroscience·F SedelA Triller
Oct 15, 1991·The Journal of Comparative Neurology·M FuscoA Leon

Citations

Mar 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·J K Brunso-Bechtold, V Hamburger
Aug 15, 1978·The Journal of Comparative Neurology·J L Dubbeldam, H J Karten
Mar 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·S A SherwinG J Todaro
May 1, 1988·Developmental Biology·D B Wayne, M B Heaton
May 1, 1985·Developmental Biology·K W Tosney, L T Landmesser
Jun 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·M TaniuchiE M Johnson
Feb 8, 1987·The Journal of Comparative Neurology·G RaivichA Sutter
Nov 15, 1987·The Journal of Comparative Neurology·M B Heaton
Oct 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·R Levi-Montalcini, L Aloe
Mar 15, 1974·Brain Research·I A HendryL L Iversen
Aug 23, 1974·Brain Research·K StöckelH Thoenen
Nov 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·A ZaniniR Levi-Montalcini
Jun 30, 1972·Science·J H LaVail, M M LaVail
Jan 1, 1980·The Journal of Comparative Neurology·M B Heaton, S A Moody
Nov 1, 1978·Neuroscience Letters·M B HeatonM E Kosier

Related Concepts

Embryo
CYCS
Mandible
Axonal Transport
Entire Brainstem
Malignant Neoplasm of Spinal Cord
Nerve Growth Factor
Trigeminal Nerve Motor Nucleus
Synovial Cyst
Neoplasm of Uncertain or Unknown Behavior of Spinal Cord

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