PMID: 8610182Apr 2, 1996Paper

The open reading frame of bamboo mosaic potexvirus satellite RNA is not essential for its replication and can be replaced with a bacterial gene

Proceedings of the National Academy of Sciences of the United States of America
N S LinY H Hsu

Abstract

A satellite RNA of 836 nt depends on the bamboo mosaic potexvirus (BaMV) for its replication and encapsulation. The BaMV satellite RNA (satBaMV) contains a single open reading frame encoding a 20-kDa nonstructural protein. A full-length infectious cDNA clone has been generated downstream of the T7 RNA polymerase promoter. To investigate the role of the 20-kDa protein encoded by satBaMV, satBaMV transcripts containing mutations in the open reading frame were tested for their ability to replicate in barley protoplasts and in Chenopodium quinoa using BaMV RNA as a helper genome. Unlike other large satellite RNAs, mutants in the open reading frame did not block their replication, suggesting that the 20-kDa protein is not essential for satBaMV replication. Precise replacement of the open reading frame with sequences encoding chloramphenicol acetyltransferase resulted in high level expression of chloramphenicol acetyltransferase in infected C. quinoa, indicating that satBaMV is potentially useful as a satellite-based expression vector.

References

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Sep 1, 1991·The Journal of General Virology·J B BancroftG A Mackie
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Sep 1, 1994·The Journal of General Virology·N S LinY H Hsu

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Citations

Sep 6, 2000·The Plant Journal : for Cell and Molecular Biology·I R ChoiR French
Jan 1, 1996·Annual Review of Phytopathology·H B ScholthofA O Jackson
Jun 10, 2008·Virus Research·Ying-Wen HuangYau-Heiu Hsu
May 17, 2013·Molecular Plant Pathology·Kuan-Yu LinNa-Sheng Lin
Nov 29, 2013·Plant Biotechnology Journal·Ming-Ru LiouYau-Heiu Hsu
May 8, 2015·Current Opinion in Virology·Ming-Ru LiouYau-Heiu Hsu
Dec 1, 2009·Viruses·Chung-Chi HuNa-Sheng Lin
Jul 10, 2019·The New Phytologist·Ying Wen HuangYau Heiu Hsu
Apr 13, 2017·Frontiers in Microbiology·Menghsiao Meng, Cheng-Cheng Lee
Jun 8, 2017·Frontiers in Microbiology·Ing-Nang WangNa-Sheng Lin
Jan 11, 2003·Biotechnology & Genetic Engineering Reviews·Claudine Porta, George P Lomonossoff
May 20, 2017·Frontiers in Microbiology·Kuan-Yu Lin, Na-Sheng Lin
Jan 15, 2009·The Journal of General Virology·Paramasivan Vijaya PalaniNa-Sheng Lin
Nov 24, 2020·Virus Genes·Uzma BadarKathleen Hefferon
May 27, 2021·Molecular Biology Reports·Stefano Decle-CarrascoEnrique Castano

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