The open reading frame TTC1157 of Thermus thermophilus HB27 encodes the methyltransferase forming N²-methylguanosine at position 6 in tRNA.

RNA
Martine RooversLouis Droogmans

Abstract

N(2)-methylguanosine (m(2)G) is found at position 6 in the acceptor stem of Thermus thermophilus tRNA(Phe). In this article, we describe the cloning, expression, and characterization of the T. thermophilus HB27 methyltransferase (MTase) encoded by the TTC1157 open reading frame that catalyzes the formation of this modified nucleoside. S-adenosyl-L-methionine is used as donor of the methyl group. The enzyme behaves as a monomer in solution. It contains an N-terminal THUMP domain predicted to bind RNA and contains a C-terminal Rossmann-fold methyltransferase (RFM) domain predicted to be responsible for catalysis. We propose to rename the TTC1157 gene trmN and the corresponding protein TrmN, according to the bacterial nomenclature of tRNA methyltransferases. Inactivation of the trmN gene in the T. thermophilus HB27 chromosome led to a total absence of m(2)G in tRNA but did not affect cell growth or the formation of other modified nucleosides in tRNA(Phe). Archaeal homologs of TrmN were identified and characterized. These proteins catalyze the same reaction as TrmN from T. thermophilus. Individual THUMP and RFM domains of PF1002 from Pyrococcus furiosus were produced. These separate domains were inactive and did not bind tRNA, rein...Continue Reading

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Citations

Mar 1, 2012·Nucleic Acids Research·Marcus FislageWim Versées
Jun 8, 2013·Journal of Molecular Biology·Rajashekhar KamalampetaUte Kothe
Jun 7, 2014·Frontiers in Genetics·Hiroyuki Hori
May 17, 2014·Nucleic Acids Research·Takeo Suzuki, Tsutomu Suzuki
Mar 7, 2007·BMC Bioinformatics·Karolina L TkaczukJanusz M Bujnicki
May 31, 2016·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Ryota YamagamiHiroyuki Hori
May 26, 2017·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Misa NakashimaHiroyuki Hori
Apr 18, 2020·Wiley Interdisciplinary Reviews. RNA·Phillip J McCownJessica A Brown

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