The OPFOS Microscopy Family: High-Resolution Optical Sectioning of Biomedical Specimens.

Anatomy Research International
Jan A N BuytaertJoris J J Dirckx

Abstract

We report on the recently emerging (laser) light-sheet-based fluorescence microscopy field (LSFM). The techniques used in this field allow to study and visualize biomedical objects nondestructively in high resolution through virtual optical sectioning with sheets of laser light. Fluorescence originating in the cross-section of the sheet and sample is recorded orthogonally with a camera. In this paper, the first implementation of LSFM to image biomedical tissue in three dimensions-orthogonal-plane fluorescence optical sectioning microscopy (OPFOS)-is discussed. Since then many similar and derived methods have surfaced, (SPIM, ultramicroscopy, HR-OPFOS, mSPIM, DSLM, TSLIM, etc.) which we all briefly discuss. All these optical sectioning methods create images showing histological detail. We illustrate the applicability of LSFM on several specimen types with application in biomedical and life sciences.

References

Sep 1, 1995·Computerized Medical Imaging and Graphics : the Official Journal of the Computerized Medical Imaging Society·A H Voie, F A Spelman
Aug 18, 2004·Journal of Microscopy·S P TinlingR S Kullar
Aug 29, 2007·European Archives of Oto-rhino-laryngology : Official Journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : Affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery·R HofmanH P Wit
Dec 10, 2008·Optics Express·C Dunsby
Mar 28, 2009·Biology Letters·Sam Van WassenberghAnthony Herrel
Apr 23, 2009·Biophysical Journal·Vladimir ErmolayevEckhard Flechsig
Jan 28, 2002·Optics Express·Eran FuchsFarooq Azam
May 20, 2009·BioTechniques·Peter A SantiJames R Leger
Mar 10, 2010·Journal of Biomedical Optics·Jerome Mertz, Jinhyun Kim
Feb 23, 2011·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Peter A Santi
Jul 14, 2011·Journal of the Association for Research in Otolaryngology : JARO·Jan A N BuytaertJoris J J Dirckx

❮ Previous
Next ❯

Citations

Jul 14, 2011·Journal of the Association for Research in Otolaryngology : JARO·Jan A N BuytaertJoris J J Dirckx
May 2, 2013·International Journal of Computer Assisted Radiology and Surgery·Thomas S RauOmid Majdani
Mar 21, 2016·International Journal of Computer Assisted Radiology and Surgery·G Jakob LexowThomas S Rau
Jan 31, 2013·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Jan A N BuytaertPeter A Santi
Jun 26, 2014·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Jan BuytaertJoris Dirckx
Feb 7, 2017·Scientific Reports·Yichen DingTzung K Hsiai
Jan 7, 2015·Current Protocols in Cytometry·Michael W AdamsJames A J Fitzpatrick
Mar 7, 2020·Journal of Cell Science·Lai WenKlaus Ley

❮ Previous
Next ❯

Methods Mentioned

BETA
fluorescence microscopy
fluorescence optical sectioning
confocal microscopy
dissection

Software Mentioned

mSPIM
OPFOS
LaVision
TSLIM
Zeiss
SPIM
LaVision BioTec

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
Peter A Santi
European Archives of Oto-rhino-laryngology : Official Journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : Affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery
Udi Cinamon
The Anatomical Record. Part B, the New Anatomist
D M L CooperB Hallgrímsson
Otolaryngology--head and Neck Surgery : Official Journal of American Academy of Otolaryngology-Head and Neck Surgery
J Sadé, Amos Ar
© 2021 Meta ULC. All rights reserved