The opgC gene is required for OPGs succinylation and is osmoregulated through RcsCDB and EnvZ/OmpR in the phytopathogen Dickeya dadantii

Scientific Reports
Sébastien Bontemps-GalloJean-Marie Lacroix

Abstract

Osmoregulated periplasmic glucans (OPGs) are a family of periplasmic oligosaccharides found in the envelope of most Proteobacteria. They are required for virulence of zoo- and phyto-pathogens. The glucose backbone of OPGs is substituted by various kinds of molecules depending on the species, O-succinyl residues being the most widely distributed. In our model, Dickeya dadantii, a phytopathogenic bacteria causing soft rot disease in a wide range of plant species, the backbone of OPGs is substituted by O-succinyl residues in media of high osmolarity and by O-acetyl residues whatever the osmolarity. The opgC gene encoding a transmembrane protein required for the succinylation of the OPGs in D. dadantii was found after an in silico search of a gene encoding a protein with the main characteristics recovered in the two previously characterized OpgC of E. coli and R. sphaeroides, i.e. 10 transmembrane segments and one acyl-transferase domain. Characterization of the opgC gene revealed that high osmolarity expression of the succinyl transferase is controlled by both the EnvZ-OmpR and RcsCDB phosphorelay systems. The loss of O-succinyl residue did not affect the virulence of D. dadantii, suggesting that only the glucose backbone of OPGs ...Continue Reading

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Aug 13, 2015·Environmental Microbiology Reports·Sébastien Bontemps-Gallo, Jean-Marie Lacroix

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Citations

Apr 4, 2017·Genome Biology and Evolution·Honour C McCannHongwen Huang
Nov 24, 2016·Environmental Microbiology·Simon LeonardSylvie Reverchon
Jun 9, 2017·EcoSal Plus·Sébastien Bontemps-GalloJean-Marie Lacroix

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Methods Mentioned

BETA
electrophoretic mobility shift assay
PCR
gel filtration

Software Mentioned

Phylip
CLUSTAL OMEGA
Dendroscope
EMBOSS Needle

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