The optical properties of rat, porcine and human lenses in organ culture treated with dexamethasone

Experimental Eye Research
Mark Bree, Douglas Borchman

Abstract

Organ culturing of lenses is useful for testing the direct effect of moieties such as cations, glucose, oxygen, antioxidants or drugs on the lens. Lens opacity changes in organ cultured lenses have never been measured quantitatively. Our aims were twofold: to establish the time course of lens opacity changes in organ cultured rat, porcine and human lenses and to test the utility of our system to measure opacity changes in lenses treated with dexamethasone. Human, porcine and rat lenses were incubated with or without 5 μM dexamethasone in minimum essential medium for 13 days. Lens absorbance and light scattering were measured using a spectrometer. Lens opacity was graded from lens photographs. Sixteen human lenses were obtained from 10 donors. Light scattering accounted for all of the absorbance of the human lens above 550 nm, 72% of the absorbance at 500 nm and 50% of the absorbance at 450 nm. Similarly, for the porcine lens, light scattering accounted for all of the absorbance above 400 nm and 40% of the absorbance at 350 nm. For the rat lens, scattering accounted for all of the absorbance above 325 nm, and 37% of the absorbance at 300 nm. In the rat lens spectrum, the broad absorbance peak near 600 nm was unique to the rat le...Continue Reading

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Citations

Jun 8, 2018·Current Eye Research·Ali Hendi Sahmi AlghamdiDouglas Borchman
Oct 29, 2020·Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes·Ana P Cuzziol BoccioniRafael C Lajmanovich
Jun 20, 2020·Journal of Lipid Research·Douglas Borchman
Feb 9, 2021·Journal of Lipid Research·Douglas Borchman

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