The optimization and production of stable homogeneous amine enriched surfaces with characterized nanotopographical properties for enhanced osteoinduction of mesenchymal stem cells

Journal of Biomedical Materials Research. Part a
Rui ChenJudith M Curran

Abstract

Silane modification has been proposed as a powerful biomaterial surface modification tool. This is the first comprehensive investigation into the effect of silane chain length on the resultant properties of -NH2 silane monolayers and the associated osteoinductive properties of the surface. A range of -NH2 presenting silanes, chain length 3-11, were introduced to glass coverslips and characterized using water contact angles, atomic force microscopy, X-ray photoelectron spectroscopy, and Ninhydrin assays. The ability of the variation in chain length to form a homogenous layer across the entirety of the surfaces was also assessed. The osteoinductive potential of the resultant surfaces was evaluated by real-time polymerase chain reaction, immunocytochemistry, and von Kossa staining. Control of surface chemistry and topography was directly associated with changes in chain length. This resulted in the identification of a specific, chain length 11 (CL11) which significantly increased the osteoinductive properties of the modified materials. Only CL11 surfaces had a highly regular nano-topography/roughness which resulted in the formation of an appetite-like layer on the surface that induced a significantly enhanced osteoinductive respon...Continue Reading

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Citations

Apr 12, 2019·International Journal of Molecular Sciences·Ines FiguereidoLaura Russo
Aug 28, 2019·Nanoscale·William LumLaura B Sagle
Mar 23, 2021·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Caroline S TaylorJohn W Haycock
Apr 23, 2020·ACS Applied Materials & Interfaces·Man LiRaechelle A D'Sa

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