The Optimized Fabrication of Nanobubbles as Ultrasound Contrast Agents for Tumor Imaging

Scientific Reports
Wen Bin CaiYun You Duan

Abstract

Nanobubbles, which have the potential for ultrasonic targeted imaging and treatment in tumors, have been a research focus in recent years. With the current methods, however, the prepared uniformly sized nanobubbles either undergo post-formulation manipulation, such as centrifugation, after the mixture of microbubbles and nanobubbles, or require the addition of amphiphilic surfactants. These processes influence the nanobubble stability, possibly create material waste, and complicate the preparation process. In the present work, we directly prepared uniformly sized nanobubbles by modulating the thickness of a phospholipid film without the purification processes or the addition of amphiphilic surfactants. The fabricated nanobubbles from the optimal phospholipid film thickness exhibited optimal physical characteristics, such as uniform bubble size, good stability, and low toxicity. We also evaluated the enhanced imaging ability of the nanobubbles both in vitro and in vivo. The in vivo enhancement intensity in the tumor was stronger than that of SonoVue after injection (UCA; 2 min: 162.47 ± 8.94 dB vs. 132.11 ± 5.16 dB, P < 0.01; 5 min: 128.38.47 ± 5.06 dB vs. 68.24 ± 2.07 dB, P < 0.01). Thus, the optimal phospholipid film thickness...Continue Reading

References

Jan 1, 1994·Ultrasound in Medicine & Biology·B B GoldbergF Forsberg
May 1, 1996·Journal of Pharmaceutical Sciences·H Alkan-OnyukselD D McPherson
Jan 1, 1997·Ultrasound in Medicine & Biology·G M LanzaS A Wickline
May 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·S K HobbsR K Jain
Apr 7, 2000·The American Journal of Pathology·H HashizumeD M McDonald
Sep 3, 2002·Advanced Drug Delivery Reviews·Irène BriggerPatrick Couvreur
Oct 24, 2002·Investigative Radiology·Patricia A SchumannTerry O Matsunaga
Mar 30, 2004·Ultrasonics·Brian E Oeffinger, Margaret A Wheatley
Mar 31, 2004·Journal of Nuclear Cardiology : Official Publication of the American Society of Nuclear Cardiology·Jonathan R Lindner
Jul 1, 2004·Circulation·Wolfgang LepperSanjiv Kaul
Feb 22, 2005·Current Opinion in Biotechnology·Xiaohu GaoShuming Nie
Mar 19, 2005·Technology in Cancer Research & Treatment·Irina V LarinaRinat O Esenaliev
Dec 21, 2005·Ultrasound in Medicine & Biology·Margaret A WheatleyBrian E Oeffinger
May 27, 2006·Science·Ralph Weissleder
Jul 12, 2007·Journal of the National Cancer Institute·Natalya RapoportAnne Kennedy
Jul 27, 2007·Annual Review of Biomedical Engineering·Katherine FerraraMark Borden
Dec 17, 2008·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·H MaedaJ Daruwalla
Jan 22, 2009·Journal of Pharmaceutical Sciences·Tsong-Long HwangJia-You Fang
Jun 26, 2009·Accounts of Chemical Research·Katherine W FerraraHua Zhang
Oct 22, 2009·Small·Ayush Verma, Francesco Stellacci
Dec 5, 2009·Molecular Pharmaceutics·Tianyi M KrupkaAgata A Exner
Dec 25, 2009·European Journal of Vascular and Endovascular Surgery : the Official Journal of the European Society for Vascular Surgery·L HermusC J Zeebregts
Feb 18, 2010·Acoustical Physics·N Ya RapoportA Kennedy
Mar 10, 2010·Journal of Biomedical Optics·Chulhong KimRonald Xu
May 14, 2010·Physics in Medicine and Biology·M B FleggC M Langton
Jun 15, 2010·Clinical Radiology·N DeshpandeJ K Willmann
Sep 21, 2010·Radiology·Stephanie R Wilson, Peter N Burns
Dec 15, 2010·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Elena Eva Julianne MarxerUdo Bakowsky
Sep 21, 2011·Nanomedicine : Nanotechnology, Biology, and Medicine·Joshua E RosenFrank X Gu
Mar 7, 2012·International Journal of Nanomedicine·Tinghui YinXintao Shuai
Aug 13, 2013·Journal of Drug Delivery·Andrew D Miller

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Citations

Jun 12, 2016·Journal of Pharmaceutical Sciences·Chen ZhouJohn F Carpenter
Aug 20, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Hengli YangYunyou Duan
Jan 20, 2017·Nature Protocols·Glen M DeLoidPhilip Demokritou
Nov 18, 2017·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Carly PellowGang Zheng
Nov 9, 2019·Journal of Ultrasound in Medicine : Official Journal of the American Institute of Ultrasound in Medicine·Yu DingHaibo Shen
Sep 27, 2019·Biological & Pharmaceutical Bulletin·Rodi AbdalkaderMitsuru Hashida
Sep 16, 2020·Biomaterials Science·Andy WijayaGuixue Wang
Jun 2, 2018·Journal of Immunology Research·Wei LvLi Zhang
Jun 20, 2019·Frontiers in Pharmacology·Jiaqi ZhangMingxing Xie
Oct 2, 2020·Nanomaterials·Gurbet KöseFabian Kiessling
Mar 18, 2021·Bioresource Technology·Wachiranon ChuenchartSamir Kumar Khanal
Oct 16, 2018·Advanced Drug Delivery Reviews·Hai Doan DoNathalie Mignet
May 12, 2021·Chemical Society Reviews·Ranhua XiongKevin Braeckmans
Jan 19, 2021·ACS Biomaterials Science & Engineering·Rajeet ChandanRinti Banerjee
Nov 2, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·Muidh AlheshibriVincent S J Craig
Feb 26, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·Zhou FangJun Hu
Feb 11, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·Ananda J Jadhav, Mostafa Barigou
Dec 12, 2018·The Journal of Physical Chemistry. B·Wei XiaoLijuan Zhang
Feb 28, 2019··Lianne H. Y. SoAl Christopher De Leon

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Methods Mentioned

BETA
dynamic light scattering
scanning electron microscopy
fluorescence microscopy
xenograft

Software Mentioned

WCIF ImageJ
SPSS
SonoVue

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