The Oral Mouse Microbiome Promotes Tumorigenesis in Oral Squamous Cell Carcinoma

MSystems
Philip StashenkoJorge Frias-Lopez

Abstract

Oral squamous cell carcinoma (OSCC) is the most common malignancy of the head and neck worldwide. Dysbiosis of the microbiome has increasingly been linked to the development of different kinds of cancer. Applying 16S rRNA gene sequence analysis and metatranscriptomic analyses, we characterized the longitudinal changes in the profiles and the function of the oral microbiome in a 4-nitroquinoline-1-oxide (4-NQO)-induced model of OSCC in gnotobiotic mice. We characterized the dynamics of the oral microbiome in this model using two different microbiome inocula: one from healthy mice and the other from mice bearing a 4-NQO-induced tumor. Mice colonized with different oral microbiomes and exposed to 4-NQO had increased tumor numbers and sizes compared to controls exposed to 4-NQO but lacking a microbiome. We observed an overall increase in diversity in the tumorigenic samples compared to that in the nontumor group not exposed to 4-NQO. Despite the variability in community dynamics, specific patterns emerged during the progression of the disease. In the two groups that were inoculated with the OSCC-associated microbiome, we observed opposite profiles of abundance in Parabacteroides and Corynebacterium While the percentage of Parabacte...Continue Reading

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Citations

Feb 25, 2020·Journal of Dental Research·Z R FitzsimondsR J Lamont
Aug 20, 2020·Journal of Dental Research·F R F TelesY Wang
Feb 2, 2021·Frontiers in Cell and Developmental Biology·Spyridon GougousisIoannis Constantinidis
Nov 17, 2020·Frontiers in Immunology·Muhammad IrfanJorge Frias-Lopez
Nov 4, 2020·International Journal of Molecular Sciences·Amel SamiC Anthony Ryan
May 1, 2021·Cancers·Se-Young ParkNa-Young Song
May 11, 2021·Frontiers in Oncology·Natarajan BhaskaranPushpa Pandiyan
Jun 7, 2021·Anaerobe·Mohammad SajidMausumi Bharadwaj
Oct 5, 2021·Frontiers in Cellular and Infection Microbiology·Jiung-Wen ChenLi-Wha Wu
Nov 12, 2020·Pathobiology : Journal of Immunopathology, Molecular and Cellular Biology·Heidi Tuominen, Jaana Rautava

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Datasets Mentioned

BETA
PRJNA549752

Methods Mentioned

BETA
PCA
Illumina sequencing
PCR
phylogenetic profiles

Software Mentioned

Quantitative Insights into Microbial Ecology ( QIIME ) package
ZEN
R package splinectomeR
imputeTS R package
Fastx clipper
Diamond
Blast2GO
HUMAnN2
REVIGO
BEDtools

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