PMID: 8041793Jul 19, 1994

The ORD1 gene encodes a transcription factor involved in oxygen regulation and is identical to IXR1, a gene that confers cisplatin sensitivity to Saccharomyces cerevisiae

Proceedings of the National Academy of Sciences of the United States of America
J R LambertM G Cumsky

Abstract

The yeast COX5a and COX5b genes encode isoforms of subunit Va of the mitochondrial inner membrane protein complex cytochrome c oxidase. These genes have been shown to be inversely regulated at the level of transcription by oxygen, which functions through the metabolic coeffector heme. In earlier studies we identified several regulatory elements that control transcriptional activation and aerobic repression of one of these genes, COX5b. Here, we report the isolation of trans-acting mutants that are defective in the aerobic repression of COX5b transcription. The mutants fall into two complementation groups. One group specifies ROX1, which encodes a product reported to be involved in transcriptional repression. The other group identified the gene we have designated ORD1. Mutations in ORD1 cause overexpression of COX5b aerobically but do not affect the expression of the hypoxic genes CYC7, HEM13, and ANB1. ORD1 mutations also do not affect the expression of the aerobic genes COX5a, CYC1, ROX1, ROX3, and TIF51A. The yeast genome contains a single ORD1 gene that resides on chromosome XI. Strains carrying chromosomal deletions of the ORD1 locus are viable and exhibit phenotypes similar to, but less severe than, that of the original mu...Continue Reading

References

Apr 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·M G CumskyRobert O Poyton
Aug 1, 1993·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·D Landsman, M Bustin
Mar 1, 1994·Trends in Genetics : TIG·R GrosschedlJ Pagel

Citations

Dec 23, 2011·Applied Microbiology and Biotechnology·Ángel Vizoso-VázquezM E Cerdán
Jan 2, 1996·Mutation Research·M M McA'Nulty, S J Lippard
Jan 20, 1999·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·R P Perez
Jun 19, 2012·Antioxidants & Redox Signaling·Jingjing Liu, Antoni Barrientos
Sep 4, 2012·Antioxidants & Redox Signaling·Myriam BourensAntoni Barrientos
Dec 17, 2008·Journal of Proteomics·Astrid BruckmannG Paul H van Heusden
Jun 5, 2008·Biochimica Et Biophysica Acta·Antoni BarrientosFlavia Fontanesi
Mar 30, 2010·FEMS Yeast Research·Raquel Castro-PregoMaría Esperanza Cerdán
Feb 14, 2018·FEMS Yeast Research·Aida Barreiro-AlonsoÁngel Vizoso-Vázquez
Dec 29, 2006·Yeast·I S Ishtar Snoek, H Yde Steensma
Feb 17, 2018·Scientific Reports·Ángel Vizoso-VázquezM E Cerdán
Jun 9, 2006·American Journal of Physiology. Cell Physiology·Flavia FontanesiAntoni Barrientos
Mar 24, 1995·The Journal of Biological Chemistry·M J CrawfordD E Goldberg

Related Concepts

IXR1 protein, S cerevisiae
NSC-119875
DNA Helix Destabilizing Proteins
DNA, Fungal
Yeast Proteins
Genes, Fungal
Calf Thymus Chromatin Protein HMG
Dioxygen
Saccharomyces cerevisiae
Transcription Factor

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