The orf13 T-DNA gene of Agrobacterium rhizogenes confers meristematic competence to differentiated cells

Plant Physiology
Pia A StiegerC Kuhlemeier

Abstract

Plant infections by the soil bacterium Agrobacterium rhizogenes result in neoplastic disease with the formation of hairy roots at the site of infection. Expression of a set of oncogenes residing on the stably integrated T-DNA is responsible for the disease symptoms. Besides the rol (root locus) genes, which are essential for the formation of hairy roots, the open reading frame orf13 mediates cytokinin-like effects, suggesting an interaction with hormone signaling pathways. Here we show that ORF13 induced ectopic expression of KNOX (KNOTTED1-like homeobox) class transcription factors, as well as of several genes involved in cell cycle control in tomato (Lycopersicon esculentum). ORF13 has a retinoblastoma (RB)-binding motif and interacted with maize (Zea mays) RB in vitro, whereas ORF13, bearing a point mutation in the RB-binding motif (ORF13*), did not. Increased cell divisions in the vegetative shoot apical meristem and accelerated formation of leaf primordia were observed in plants expressing orf13, whereas the expression of orf13* had no influence on cell division rates in the shoot apical meristem, suggesting a role of RB in the regulation of the cell cycle in meristematic tissues. On the other hand, ectopic expression of L...Continue Reading

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Citations

Sep 25, 2007·The Plant Cell·Shinji TerakuraYasunori Machida
Jan 14, 2012·Trends in Plant Science·Ruben GutzatWilhelm Gruissem
Mar 2, 2006·The Plant Journal : for Cell and Molecular Biology·Bernadette ClémentLéon Otten
Feb 20, 2015·Journal of Plant Research·Masaki Ito, Yasunori Machida
Oct 9, 2016·Plant Science : an International Journal of Experimental Plant Biology·Nete KodahlHenrik Lütken
Jul 8, 2017·PloS One·Yordan S YordanovVictor B Busov
Oct 3, 2017·Frontiers in Plant Science·Ke Chen, Léon Otten
Jan 15, 2021·Proceedings of the National Academy of Sciences of the United States of America·Savio D RodriguesLaurens Pauwels

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