The ORF3 protein of porcine circovirus type 2 is involved in viral pathogenesis in vivo.

Journal of Virology
Jue LiuJimmy Kwang

Abstract

Porcine circovirus type 2 (PCV2) is the primary causative agent of an emerging swine disease, postweaning multisystemic wasting syndrome. We previously showed that a novel identified protein, ORF3, was not essential for PCV2 replication in cultured PK15 cells and played a major role in virus-induced apoptosis. To evaluate the role of the ORF3 protein in viral pathogenesis in vivo, we inoculated 8-week-old BALB/c mice that have been developed for PCV2 replication with ORF3-deficient mutant PCV2 (mPCV2). By 42 days postinoculation, all of the mice inoculated with the mPCV2, as well as with wild-type PCV2 (wPCV2), had seroconverted to PCV2 capsid antibody, and the mutant induced levels of PCV2 antibodies that were higher than those of the wPCV2. The PCV2 genomic copy numbers in serum were significantly higher (P<0.05) in the wPCV2-inoculated mice than in mice inoculated with the mPCV2. Also, the wPCV2 caused microscopic lesions characterized by lymphocyte depletion with histiocytic infiltration of lymphoid organs, but the mutant virus failed to induce any obvious pathological lesions. In situ hybridization and immunohistochemical analyses also showed that larger amounts of viral DNA and antigens were detected in the lymph nodes of...Continue Reading

References

Jan 1, 1986·Archives of Virology·I TischerW Griem
Jun 25, 1994·The Veterinary Record·S Edwards, J J Sands
Jan 1, 1994·Journal of Virology·M H NotebornG Koch
Feb 24, 1998·The Journal of General Virology·A MankertzH J Buhk
Apr 4, 1998·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·G M AllanB M Adair
Jul 31, 1998·Veterinary Pathology·M KiupelD M Haines
Sep 25, 1998·The Journal of General Virology·B M MeehanG M Allan
Jan 30, 1999·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·J EllisF McNeilly
Nov 5, 1999·Annual Review of Microbiology·A RoulstonP E Branton
Nov 24, 1999·The Journal of Veterinary Medical Science·A OnukiH Tsunemitsu
Feb 2, 2000·Virus Research·A MankertzD Soike
Feb 26, 2000·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·G M Allan, J A Ellis
Mar 24, 2000·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·C ChoiE G Clark
Aug 22, 2000·The Journal of General Virology·P NawagitgulP S Paul
Jun 12, 2002·Veterinary Research·Josefina QuintanaMariano Domingo
Oct 29, 2002·The Veterinary Quarterly·J Segalés, M Domingo
Dec 31, 2002·Virology·Andrew K Cheung
May 6, 2003·Veterinary Immunology and Immunopathology·J NielsenK C McCullough
Apr 27, 2004·The Journal of Veterinary Medical Science·Changming LiuSusumu Ueda
May 12, 2004·Current Opinion in Immunology·Raymond M WelshXiaoting Z Wang

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Citations

Feb 1, 2012·Archives of Virology·Van Giap NguyenBong Kyun Park
Jun 12, 2012·Virologica Sinica·Xing-xiao ZhangShi-jin Jiang
Jun 30, 2012·Hybridoma·Jia-Ling HeJi-Yong Zhou
Oct 13, 2007·Viral Immunology·Leanne S StevensonGordon M Allan
Sep 3, 2010·Journal of Virology·Mark ChaiyakulMarkus Czub
Mar 16, 2012·Virology Journal·Xin-sheng LiuYong-lu Wang
Jul 12, 2014·Virus Genes·Qi-Zhuang LvYan-Ming Zhang
Apr 2, 2011·Research in Veterinary Science·Mihai A TurcituRaluca Cioranu
Apr 2, 2016·Research in Veterinary Science·Haiyan WangGuoqing Shao
Jan 27, 2016·International Journal of Biological Macromolecules·Zhiyuan PengLinzhu Ren
Apr 28, 2012·Veterinary Microbiology·Qi-Wang XiangShi-Jin Jiang
Dec 20, 2011·Virus Research·Laila Darwich, Enric Mateu
Nov 16, 2010·Research in Veterinary Science·Avanti SinhaTanja Opriessnig
Aug 17, 2010·Journal of Comparative Pathology·I Galindo-CardielJ Segalés
Mar 27, 2016·Virus Genes·Linzhu RenHongsheng Ouyang

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