PMID: 3756470Aug 13, 1986Paper

The organization of intrinsic hippocampal connections in explants of rat hippocampus studied by topical application of HRP crystals

Brain Research
F KellerP G Waser

Abstract

Hippocampal slices were taken from 7-day-old rats and maintained in vitro for 1-3 weeks. The organization of intrahippocampal connections in these explants was studied by placing onto the tissue small crystals of horseradish peroxidase (HRP) soaked with the detergent Nonidet. Antero- and retrograde transport of HRP was visualized by diaminobenzidine. The principal arrangement of intrinsic hippocampal connections closely resembles the in situ situation of the adult rat. The use of HRP crystals provides a fast and convenient tool for the study of connections in brain explants.

References

Apr 6, 1979·Anatomy and Embryology·D G Amaral
Oct 15, 1978·The Journal of Comparative Neurology·L W SwansonW M Cowan
Sep 20, 1984·The Journal of Comparative Neurology·J Zimmer, B H Gähwiler

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Citations

Jan 1, 1987·Acta Neuropathologica·K RimvallP G Waser
May 1, 1989·Brain Research·N Bousez-DumesnilY Ben-Ari
Dec 1, 1993·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·I SchousboeA Schousboe
Apr 28, 2001·Journal of Neuroscience Methods·G MarínJ Mpodozis

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