Jul 5, 1993

The organo-specific external NADH dehydrogenase of mammal heart mitochondria has an artefactual origin

Biochimica Et Biophysica Acta
L FraisseM Rigoulet

Abstract

The existence of an organo-specific (heart) external NADH dehydrogenase located on the outer face of the inner mitochondrial membrane has been recently proposed. We have studied the respiration on external NADH in rat and beef heart mitochondrial fractions: (i) by using different mitochondrial isolation procedures on the rat, we observed that the higher the criteria of quality toward classical substrate respiration of mitochondrial fractions, the lower the external NADH-linked respiration; (ii) by using an especially loosely fitting glass-Teflon homogenizer, we obtained rat heart mitochondrial fractions practically free from external NADH linked respiration and with the highest respiratory control ratio on glutamate plus malate respiration. In rat and beef heart mitochondrial fractions containing an external NADH respiration: (i) ethoxyformic anhydride used previously to distinguish internal and external NADH oxidation was shown not to be specific; (ii) external NADH-linked respiration (although associated to the normally functioning respiratory chain as was shown by the effects of classic respiratory inhibitors) did not lead to ADP phosphorylation while glutamate plus malate did; (iii) respiratory activity on glutamate plus ma...Continue Reading

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Citations

Mentioned in this Paper

Cytochrome C Oxidase
Cytochrome c Oxidase Subunit VIa
Beef heart preparation
Teflon
Cell Respiration
Mitochondria, Heart
Respiratory System
NADH
Polytetrafluoroethylene
Oxidation

About this Paper

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