PMID: 9159137May 27, 1997Paper

The origin and efficient derivation of embryonic stem cells in the mouse

Proceedings of the National Academy of Sciences of the United States of America
Frances A Brook, Richard L Gardner

Abstract

By explanting tissues isolated microsurgically from implanting strain 129 mouse blastocysts individually on STO feeder cells we have established that embryonic stem (ES) cells originate from the epiblast (primitive ectoderm). Isolated early epiblasts yielded ES cell lines at a substantially higher frequency than intact blastocysts regardless of whether they were explanted whole or as strictly single-cell suspensions. When explanted from delayed-implanting 129 blastocysts, epiblasts gave lines consistently in 100% of cases. If primary embryonic fibroblasts rather than STO cells were used as feeders, germline-competent ES cell lines were obtained readily from epiblasts of delayed-implanting blastocysts of several hitherto refractory strains, particularly when recombinant leukemia inhibitory factor was included in the medium during the initial period of culture. Because lines were obtained from the nonpermissive CBA/Ca strain at a rate of up to 56%, this approach to the derivation of germline-competent ES cell lines may not only prove generic for the mouse but also worth pursuing in other species of mammal.

References

Apr 15, 1974·International Journal of Cancer. Journal International Du Cancer·H Sobis, M Vandeputte
Dec 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·G R Martin
Jan 1, 1993·Methods in Enzymology·S J AbbondanzoC L Stewart

❮ Previous
Next ❯

Citations

Jun 17, 1998·Developmental Dynamics : an Official Publication of the American Association of Anatomists·J W XiongH Stuhlmann
Aug 2, 2003·Medical & Biological Engineering & Computing·E RocheB Soria
May 10, 2012·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Salvador Aznar Benitah, Michaela Frye
Aug 12, 2005·Cell and Tissue Research·Catherine M BrowneKate L Loveland
Sep 24, 2010·Journal of Assisted Reproduction and Genetics·Sheyla GonzálezJosep Santaló
Jan 17, 2009·In Vitro Cellular & Developmental Biology. Animal·Anne S JürgensJürgen S Scheele
Mar 25, 2010·In Vitro Cellular & Developmental Biology. Animal·Neil C TalbotLouis S Stodieck
Jul 8, 2010·In Vitro Cellular & Developmental Biology. Animal·Neil C TalbotThomas J Caperna
Dec 20, 2011·In Vitro Cellular & Developmental Biology. Animal·Neil C TalbotThomas J Caperna
Dec 20, 2011·In Vitro Cellular & Developmental Biology. Animal·Peng ZhangXiangyun Li
Dec 5, 2006·Stem Cell Reviews·Jürgen HeschelerJohannes Winkler
Mar 8, 2005·Reproductive Toxicology·Huaping ZhangZhongdong Qiao
Sep 21, 2013·Stem Cell Reports·Harry G LeitchAustin Smith
May 21, 2013·Trends in Cell Biology·Maaike Welling, Niels Geijsen
May 6, 2003·Developmental Biology·Myriam HembergerJames C Cross
Mar 1, 2003·Frontiers in Neuroendocrinology·Sara Wells, David Murphy
Jun 16, 2000·Neuroscience Letters·G A ElderR A Lazzarini
Sep 5, 2001·Current Opinion in Genetics & Development·J Rathjen, P D Rathjen
Dec 15, 2000·Current Biology : CB·P J FairchildH Waldmann
Dec 24, 2002·Comptes rendus biologies·Martin Evans, Susan Hunter
Jan 11, 2007·Nature Biotechnology·Thomas Gridley, Rick Woychik
Jun 7, 2013·Nature Communications·Tomokazu AmanoMinoru S H Ko
Feb 19, 2013·Nature Structural & Molecular Biology·Harry G LeitchPetra Hajkova
Sep 26, 2002·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Kunio ShiotaNaka Hattori
May 30, 2002·Journal of Anatomy·R L Gardner
Mar 19, 2002·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·D L Stocum
Mar 19, 2002·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·K S O'Shea
Jul 24, 2012·Molecular & Cellular Proteomics : MCP·Jing SongRob M Ewing
Jan 27, 2005·Cloning and Stem Cells·Ian Chambers
May 25, 2007·Stem Cells and Development·Atindriya Biswas, Robert Hutchins
Nov 16, 2006·Stem Cells and Development·Stuart AveryHarry Moore
Jan 27, 2007·Stem Cells and Development·Nicole L WollSarah K Bronson
Feb 15, 2008·Stem Cells and Development·Chang Hyun LeeSe Pill Park

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.