Apr 5, 2020

The origin and evolution of a distinct mechanism of transcription initiation in yeasts

BioRxiv : the Preprint Server for Biology
Zhaolian Lu, Zhenguo Lin


The molecular process of transcription by RNA Polymerase II is highly conserved among eukaryotes ("classic model"). Intriguingly, a distinct way of locating transcription start sites (TSSs) was found in a budding yeast Saccharomyces cerevisiae ("scanning model"). The origin of the "scanning model" and its underlying genetic mechanisms remain unsolved. Herein, we applied genomic approaches to address these questions. We first identified TSSs at a single-nucleotide resolution for 12 yeast species using the nAnT-iCAGE technique, which significantly improved the annotations of these genomes by providing accurate 5'-boundaries of protein-coding genes. We then infer the initiation mechanism of a species based on its TSS maps and genome sequences. We found that the "scanning model" had originated after the split of Yarrowia lipolytica and the rest of budding yeasts. An adenine-rich region immediately upstream of TSS had appeared during the evolution of the "scanning model" species, which might facilitate TSS selection in these species. Both initiation mechanisms share a strong preference for pyrimidine-purine dinucleotides surrounding the TSS. Our results suggested that the purine is required for accurately recruiting the first nucleo...Continue Reading

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Mentioned in this Paper

Protein Complex Subunit Gene
Wright stain
Diffusion Weighted Imaging
Complex (molecular entity)
Recombination, Genetic
Genetic Inheritance

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