PMID: 9421067Jan 8, 1998Paper

The origin and molecular characterization of adenoid basal carcinoma of the uterine cervix

International Journal of Gynecological Pathology : Official Journal of the International Society of Gynecological Pathologists
M W JonesS D Finkelstein

Abstract

Five cases of adenoid basal carcinoma (ABC) of the uterine cervix were examined for the presence of p53 tumor suppressor gene, K-ras-2 oncogene, and human papillomavirus (HPV). A topographic genotyping approach was used to search for point mutations in K-ras-2 (exon 1 and 2) and p53 (exons 5 to 8) in archival formalin-fixed tissue blocks. Minute target sites were selected from polymerase chain reaction (PCR) amplified and directly sequenced tissue sections. Tissue sections were additionally subjected to immunohistochemical staining for p53 and WAF-1 protein. Because wild type p53 induces WAF-1 gene expression, immunohistochemical staining for WAF-1 protein using monoclonal antibodies may serve as an indirect means to test for p53 mutational damage. Mutational genotype was compared to histopathologic features and immunohistochemical staining. To study the role of HPV, L1 region consensus primers were used to amplify topographic samples, followed by HPV genotyping by direct sequencing and comparison to known viral strains. ABC was found to contain HPV in all cases, proven by genotyping to be HPV type 16 in each case. The virus showed no evidence of genomic variation from prototype HPV type 16 in the L1 segment examined. No K-ras-...Continue Reading

Citations

Jun 19, 2007·Medical Molecular Morphology·Naoto KurodaHideaki Enzan
Feb 19, 2000·The American Journal of Surgical Pathology·W Grayson, K Cooper
Mar 28, 2002·International Journal of Gynecological Pathology : Official Journal of the International Society of Gynecological Pathologists·Yukio TakeshimaKouki Inai
Sep 28, 2002·International Journal of Gynecological Pathology : Official Journal of the International Society of Gynecological Pathologists·William R Hart
Sep 28, 2002·International Journal of Gynecological Pathology : Official Journal of the International Society of Gynecological Pathologists·Michael Wells, Laurence J R Brown
Aug 27, 2002·Advances in Anatomic Pathology·Wayne Grayson, Kumarasen Cooper
Aug 17, 2006·Diagnostic Pathology·Michael J Russell, Oluwole Fadare
Aug 18, 2006·Diagnostic Pathology·William David DePondKristyn Poncy Menendez
Sep 10, 2005·Best Practice & Research. Clinical Obstetrics & Gynaecology·A J Tiltman
Dec 10, 2015·The American Journal of Surgical Pathology·Deyin XingBrigitte M Ronnett
Sep 10, 2015·International Journal of Gynecological Pathology : Official Journal of the International Society of Gynecological Pathologists·Abha GoyalBin Yang
Jun 25, 2004·Archives of Pathology & Laboratory Medicine·Haytham H DimashkiehMary B Casey
Oct 23, 2020·Archives of Pathology & Laboratory Medicine·Laurie M GriesingerStephanie L Skala

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