The origin of greater-than-unit-length plasmids generated during bacterial conjugation

Molecular Microbiology
M J Erickson, R J Meyer

Abstract

In Gram-negative bacteria, the general mechanism of conjugal plasmid transfer, which is probably similar for many different groups of plasmids, involves the transfer of a single plasmid DNA strand with 5' to 3' polarity. Transfer is initiated by nicking of the duplex DNA at a particular site, i.e. the origin of transfer (oriT). We constructed plasmids containing two directly repeated copies of oriT, derived from the broad-host-range plasmid R1162 and flanking the lac operator. The number of lacO copies in the plasmid after transfer could be determined from the colour of transconjugant colonies on medium containing X-Gal. When the oriTs were mutated to prevent initiation and termination of transfer at the same oriT, almost all of the transconjugant cells contained greater-than-unit-length plasmids with two copies of lacO and three copies of oriT. We show that these molecules were generated by an intramolecular, conjugation-dependent mechanism unlikely to depend solely on a pre-existing population of circular or linear multimers in donor cells. We propose that the greater-than-unit-length molecules were instead generated by a rolling-circle mechanism of DNA replication.

References

Oct 1, 1992·Journal of Bacteriology·M BhattacharjeeR J Meyer
Jun 5, 1989·Journal of Molecular Biology·T L ThompsonR C Deonier
Jan 1, 1987·Molecular & General Genetics : MGG·K M Derbyshire, N S Willetts
Jul 1, 1986·Journal of Bacteriology·A Cohen, A J Clark
Aug 1, 1986·Journal of Bacteriology·M A Brasch, R J Meyer
May 1, 1974·Journal of Medical Microbiology·R S Jobanputra, N Datta
Sep 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·V HershfieldD R Helinski
Jan 1, 1968·Cold Spring Harbor Symposia on Quantitative Biology·W D Rupp, G Ihler
Jan 1, 1968·Cold Spring Harbor Symposia on Quantitative Biology·M Ohki, J Tomizawa
May 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·G Ihler, W D Rupp
Jun 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·E M WongB Polisky
May 1, 1982·Journal of Bacteriology·R MeyerM Brasch
Jul 1, 1983·Analytical Biochemistry·A P Feinberg, B Vogelstein
Jun 1, 1981·Genetical Research·N Willetts, C Crowther
Nov 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·G J Warren, A J Clark
Jan 1, 1981·Molecular & General Genetics : MGG·A Laban, A Cohen
Nov 15, 1981·Analytical Biochemistry·P Stougaard, S Molin

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Citations

Jul 1, 1995·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·J A Shapiro
Jan 1, 1995·Advances in Biophysics·T KomanoT Yoshida
Jun 7, 2005·Journal of Bacteriology·Gustavo SantoyoDavid Romero
Jul 17, 2007·Proceedings of the National Academy of Sciences of the United States of America·Scott A LujanMatthew R Redinbo
Jul 1, 2017·Nihon saikingaku zasshi. Japanese journal of bacteriology·Yasuyoshi Ike
Feb 1, 1994·Molecular Microbiology·Q GaoR C Deonier
Feb 12, 2015·FEMS Microbiology Reviews·Anne Mai-ProchnowScott A Rice
Jul 25, 2000·Journal of Molecular Biology·E C Becker, R J Meyer
Mar 15, 1996·Genes & Development·G Blakely, D Sherratt

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