The origin recognition complex is dispensable for endoreplication in Drosophila.

Proceedings of the National Academy of Sciences of the United States of America
So Young Park, Maki Asano

Abstract

The origin recognition complex (ORC) is an essential component of the prereplication complex (pre-RC) in mitotic cell cycles. The role of ORC as a foundation to assemble the pre-RC is conserved from yeast to human. Furthermore, in metazoans ORC plays a key role in determining the timing of replication initiation and origin usage. In this report we have produced and analyzed a Drosophila orc1 allele to investigate the roles of ORC1 in three different modes of DNA replication during development. As expected, ORC1 is essential for mitotic replication and proliferation in brains and imaginal discs, as well as for gene amplification in ovarian follicle cells. Surprisingly, however, ORC1 is not required for endoreplication. Decreased cell number in orc1 mutant salivary glands is consistent with the idea that undetectable levels of maternal ORC1 during embryogenesis fail to support further proliferation. Nevertheless, these cells begin endoreplicating normally and reach a final ploidy of >1000C in the absence of zygotic synthesis of ORC1. The dispensability of ORC is further supported by an examination of other ORC members, whereas Double-parked protein/Cdt1 and minichromosome maintenance proteins are apparently essential for endorepl...Continue Reading

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Jun 23, 2009·Proceedings of the National Academy of Sciences of the United States of America·Maxim BalasovIgor Chesnokov
Mar 24, 2010·Proceedings of the National Academy of Sciences of the United States of America·Jianhong WuTao-shih Hsieh
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