PMID: 12770299May 29, 2003Paper

The origin, transport and cleavage of the molt-associated cuticular protein CECP22 from Calpodes ethlius (Lepidoptera, Hesperiidae)

Journal of Insect Physiology
O Marcu, M LOCKE

Abstract

CECP22 (Calpodes ethlius Cuticular Protein 22 kDa) is a molt associated protein found in the cuticle of C. ethlius larvae and pupae. The mRNA for the CECP22 cuticular protein is expressed in the epidermis and fat body during the intermolt. The protein itself accumulates in intermolt hemolymph, but at molting, when the cuticle is being digested, it is also found in the cuticle of surface integument, tracheae, foregut and hindgut and in the molting fluid. CECP22 exists in two forms. The large form (19.17 kDa, pI 6.2) becomes smaller (16.1 kDa, pI 7.4) by cleavage at the proteolytic cleavage site (position 170) with amidation of the C-terminal. The small, more basic peptide, appears only at molting, first in the cuticle and then in the molting fluid. It is presumed to be the active form of an amidase involved in the earliest stages of cuticle degradation. The inactive form accumulates in the hemolymph during the long intermolt and probably represents an abundant source of precursor enzyme that can be provided to all cuticle containing organs for a precise initiation of cuticle degradation.

References

Sep 29, 1978·Biochemical and Biophysical Research Communications·M L Bade, A Stinson
Oct 30, 1996·Molecular and Cellular Endocrinology·A Martinez, A M Treston
Oct 2, 1998·Insect Biochemistry and Molecular Biology·O Marcu, M Locke

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Citations

Oct 2, 1998·Insect Biochemistry and Molecular Biology·O Marcu, M Locke
Aug 27, 2002·Annual Review of Entomology·Michael Locke

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