Abstract
The purpose of this study was to evaluate and compare the osteoblastic differentiation ability of dedifferentiated fat (DFAT) cells and adipose stem cells (ASCs) from the buccal fat pad (BFP). We isolated human DFAT cells and ASCs from the BFP of a patient who underwent oral and maxillofacial surgery and then analyzed their cell surface antigens by flow cytometry. Then, the cells were cultured in osteogenic medium for 14 days. Measurement of bone-specific alkaline phosphatase (BAP), osteocalcin (OCN), and calcium deposition and alizarin red staining were performed to evaluate the osteoblastic differentiation ability of both cell types. ASCs and DFAT cells were positive for CD90 and CD105 and negative for CD11b, CD34, and CD45. BAP (days 3 and 7), OCN (day 14), and calcium deposition (days 7 and 14) within DFAT cell cultures were significantly higher than those in ASC cultures. The alizarin red-stained area in DFAT cell cultures, which indicates mineralized matrix deposition, was stained more strongly than that in ASC cultures. The cell surface antigens of ASCs and DFAT cells tend to be similar. Furthermore, the osteoblastic differentiation ability of human DFAT cells is higher than that of ASCs from the BFP. Isolation of DFAT c...Continue Reading
References
Jan 1, 1992·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·J B Lian, G S Stein
Apr 1, 1997·The British Journal of Oral & Maxillofacial Surgery·R Martín-GranizoF Diaz
Apr 17, 2001·Tissue Engineering·P A ZukM H Hedrick
Dec 12, 2002·Molecular Biology of the Cell·Patricia A ZukMarc H Hedrick
Jan 7, 2004·Biochemical and Biophysical Research Communications·Rei OgawaHiko Hyakusoku
Sep 11, 2004·Biochemical and Biophysical Research Communications·Ken YagiKoichiro Kano
Jun 16, 2005·International Journal of Oral and Maxillofacial Surgery·A AbuabaraR W F Moreira
Jul 28, 2005·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Hidemi HattoriMasayuki Ishihara
Mar 30, 2007·Biochemical and Biophysical Research Communications·Huiwu LiJueren Lou
Apr 11, 2007·Stem Cells·Andreas Schäffler, Christa Büchler
May 24, 2007·Tissue Engineering·Eulsik YoonGregory R D Evans
Nov 6, 2007·Histochemistry and Cell Biology·Yunfeng LinWeidong Tian
Dec 8, 2007·Journal of Cellular Physiology·Taro MatsumotoHideo Mugishima
Jul 4, 2008·Journal of Periodontology·Sally A El HaddadMohammad El Shall
Mar 10, 2009·Journal of Bioscience and Bioengineering·Jiyuan ZhaoTeruyuki Nagamune
Jan 19, 2010·Tissue Engineering. Part C, Methods·Elisabet Farré-GuaschNúria Casals
Jan 13, 2012·Molecular Biology Reports·Qian GaoGongshe Yang
Mar 1, 2012·Stem Cells·Antonella PoloniSaverio Cinti
Mar 15, 2012·Stem Cells·Hiroshi MizunoA Cagri Uysal
Apr 25, 2012·Cytotechnology·Naotaka KishimotoJunichiro Kotani
Apr 28, 2012·Journal of Dental Research·T ShiraishiI Asahina
Dec 7, 2013·Cytotechnology·Fumito SakamotoNaoyuki Matsumoto
Citations
Feb 6, 2016·International Journal of Oral and Maxillofacial Surgery·A Khojasteh, N Sadeghi
Mar 5, 2016·Frontiers in Physiology·Daisuke AkitaMasaki Honda
Dec 15, 2015·Tissue Engineering. Part C, Methods·Niina TsurumachiMasaki Honda
Nov 26, 2015·International Journal of Molecular Sciences·Koji KaidaShunsuke Baba
Sep 24, 2015·Clinical Oral Investigations·Manal FareaKasmawati Mokhtar
Feb 12, 2015·Tissue Engineering. Part a·Naoya OkitaNaoyuki Matsumoto
Feb 27, 2018·Journal of Oral Science·Niina TsurumachiMasaki Honda
Jan 23, 2018·Oral Diseases·N KishimotoS D Tran
Jan 21, 2018·Cytotechnology·Tomoki KakudoYoshihiro Momota
Jul 11, 2019·Cell and Tissue Research·Julie Anne CôtéAndré Tchernof
Oct 22, 2019·Journal of Oral Science·Atsushi TatenoMasaki Honda
Jun 11, 2019·Plastic and Reconstructive Surgery·Yoshitaro SasaharaNobuyuki Mitsukawa
Jun 21, 2016·Organogenesis·Mickey ShahGe Zhang
Aug 29, 2019·Plastic and Reconstructive Surgery·Yoshitaro SasaharaNobuyuki Mitsukawa
Aug 2, 2017·Stem Cells International·Nasim Salehi-NikArash Khojasteh
Dec 14, 2017·International Journal of Molecular Sciences·Marco SalerGiulia Gastaldi
Apr 23, 2019·Clinical Hemorheology and Microcirculation·Zijun DengAndreas Lendlein
Dec 7, 2018·International Journal of Molecular Sciences·Satoshi SasayamaShunsuke Baba
Nov 18, 2020·Materials·Kosuke NakanoYoshiya Hashimoto