The osteoblastic differentiation ability of human dedifferentiated fat cells is higher than that of adipose stem cells from the buccal fat pad

Clinical Oral Investigations
Naotaka KishimotoJunichiro Kotani

Abstract

The purpose of this study was to evaluate and compare the osteoblastic differentiation ability of dedifferentiated fat (DFAT) cells and adipose stem cells (ASCs) from the buccal fat pad (BFP). We isolated human DFAT cells and ASCs from the BFP of a patient who underwent oral and maxillofacial surgery and then analyzed their cell surface antigens by flow cytometry. Then, the cells were cultured in osteogenic medium for 14 days. Measurement of bone-specific alkaline phosphatase (BAP), osteocalcin (OCN), and calcium deposition and alizarin red staining were performed to evaluate the osteoblastic differentiation ability of both cell types. ASCs and DFAT cells were positive for CD90 and CD105 and negative for CD11b, CD34, and CD45. BAP (days 3 and 7), OCN (day 14), and calcium deposition (days 7 and 14) within DFAT cell cultures were significantly higher than those in ASC cultures. The alizarin red-stained area in DFAT cell cultures, which indicates mineralized matrix deposition, was stained more strongly than that in ASC cultures. The cell surface antigens of ASCs and DFAT cells tend to be similar. Furthermore, the osteoblastic differentiation ability of human DFAT cells is higher than that of ASCs from the BFP. Isolation of DFAT c...Continue Reading

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Citations

Dec 15, 2015·Tissue Engineering. Part C, Methods·Niina TsurumachiMasaki Honda
Nov 26, 2015·International Journal of Molecular Sciences·Koji KaidaShunsuke Baba
Feb 27, 2018·Journal of Oral Science·Niina TsurumachiMasaki Honda
Jul 11, 2019·Cell and Tissue Research·Julie Anne CôtéAndré Tchernof
Jun 21, 2016·Organogenesis·Mickey ShahGe Zhang
Aug 2, 2017·Stem Cells International·Nasim Salehi-NikArash Khojasteh
Dec 14, 2017·International Journal of Molecular Sciences·Marco SalerGiulia Gastaldi
Apr 23, 2019·Clinical Hemorheology and Microcirculation·Zijun DengAndreas Lendlein

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Methods Mentioned

BETA
flow cytometry

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