The Osteocyte Transcriptome Is Extensively Dysregulated in Mouse Models of Osteogenesis Imperfecta

JBMR Plus
Sarah M ZimmermanRoy Morello

Abstract

Osteocytes are long-lived, highly interconnected, terminally differentiated osteoblasts that reside within mineralized bone matrix. They constitute about 95% of adult bone cells and play important functions including in the regulation of bone remodeling, phosphate homeostasis, and mechanical stimuli sensing and response. However, the role of osteocytes in the pathogenesis of congenital diseases of abnormal bone matrix is poorly understood. This study characterized in vivo transcriptional changes in osteocytes from CrtapKO and oim/oim mouse models of osteogenesis imperfecta (OI) compared with wild-type (WT) control mice. To do this, RNA was extracted from osteocyte-enriched cortical femurs and tibias, sequenced and subsequently analyzed to identify differentially expressed transcripts. These models were chosen because they mimic two types of OI with different genetic mutations that result in distinct type I collagen defects. A large number of transcripts were dysregulated in either model of OI, but 281 of them were similarly up- or downregulated in both compared with WT controls. Conversely, very few transcripts were differentially expressed between the CrtapKO and oim/oim mice, indicating that distinct alterations in type I col...Continue Reading

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Citations

Mar 13, 2020·JBMR Plus·Teresita Bellido, Jesus Delgado-Calle
Aug 1, 2020·Frontiers in Endocrinology·Janak L PathakJenneke Klein-Nulend
Nov 7, 2020·The Journal of Biological Chemistry·Shinji TakeyariKeiichi Ozono
Jan 8, 2021·International Journal of Molecular Sciences·Luca BiniLaura Bianchi
Jan 19, 2021·The Journal of Biological Chemistry·Shinji TakeyariKeiichi Ozono
Sep 28, 2020·Cellular Signalling·Julia EtichFrank Zaucke
Apr 4, 2021·Bone·Pierre MoffattFrank Rauch
May 1, 2021·International Journal of Molecular Sciences·Matthias MährNadja Fratzl-Zelman
Jun 3, 2021·International Journal of Molecular Sciences·Pierre MoffattFrank Rauch
Nov 11, 2021·Current Osteoporosis Reports·Natalie Ky WeeRoy Morello
Aug 3, 2021·Physiological Reviews·Jesus Delgado-Calle, Teresita Bellido

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Methods Mentioned

BETA
RNAseq
PCR
scraping

Software Mentioned

WebGestalt
Database for Annotation , Visualization , and Integrated Disco...
Illumina
Fragment Analyzer
DAVID
DESeq2
Bowtie
Seq QC Plot
BiNGO
TopHat

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