The osteogenic response of mesenchymal stromal cells to strontium-substituted bioactive glasses

Journal of Tissue Engineering and Regenerative Medicine
Martin E Santocildes-RomeroCheryl A Miller

Abstract

Bioactive glasses are known to stimulate bone healing, and the incorporation of strontium has the potential to increase their potency. In this study, calcium oxide in the 45S5 bioactive glass composition was partially (50%, Sr50) or fully (100%, Sr100) substituted with strontium oxide on a molar basis. The effects of the substitution on bioactive glass properties were studied, including density, solubility, and in vitro cytotoxicity. Stimulation of osteogenic differentiation was investigated using mesenchymal stromal cells obtained from rat bone marrow. Strontium substitution resulted in altered physical properties including increased solubility. Statistically significant reductions in cell viability were observed with the addition of bioactive glass powders to culture medium. Specifically, addition of ≥ 13.3 mg/ml of 45S5 bioactive glass or Sr50, or ≥ 6.7 mg/ml of Sr100, resulted in significant inhibition. Real-time PCR analyses detected the upregulation of genes associated with osteoblastic differentiation in the presence of all bioactive glass compositions. Some genes, including Alpl and Bglap, were further stimulated in the presence of Sr50 and Sr100. It was concluded that strontium-substituted bioactive glasses promoted os...Continue Reading

References

Nov 1, 1988·Cell and Tissue Research·C ManiatopoulosA H Melcher
Oct 31, 2001·Calcified Tissue International·P J MarieC Rey
Sep 7, 2004·Journal of Materials Science. Materials in Medicine·K E WallaceP V Hatton
Jan 1, 2005·Biomaterials·Michela Bosetti, Mario Cannas
Feb 5, 2005·Journal of Biomedical Materials Research. Part a·S RadinP Ducheyne
Sep 27, 2005·Current Opinion in Pharmacology·Pierre J Marie
Nov 24, 2006·Journal of Materials Science. Materials in Medicine·Larry L Hench
Nov 27, 2007·Journal of Tissue Engineering and Regenerative Medicine·Jun-Ying SunDavid C Greenspan
Mar 12, 2008·Archives of Biochemistry and Biophysics·Pierre J Marie
Jan 28, 2009·Journal of Biomedical Materials Research. Part a·Alejandro A GorustovichJosé M Porto López
Mar 4, 2009·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Songlin PengWilliam W Lu
Jul 24, 2012·Pharmacology & Therapeutics·Zuzana Saidak, Pierre J Marie
Aug 28, 2012·Acta Biomaterialia·Julian R Jones
May 9, 2014·Osteoporosis International : a Journal Established As Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·L WeiY Zhang

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Citations

Mar 10, 2016·Materials Science & Engineering. C, Materials for Biological Applications·J Carlos AlmeidaM Helena R Fernandes
Sep 11, 2016·Materials Science & Engineering. C, Materials for Biological Applications·Sampath Kumar Arepalli S P Singh
Aug 17, 2016·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Ana F BritoJosé M F Ferreira
Jun 22, 2017·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Isabelle DenryJulie A Holloway
Jan 23, 2019·Journal of Tissue Engineering and Regenerative Medicine·Uresha PatelVirginie Sottile
Aug 4, 2019·Journal of Biomedical Materials Research. Part a·Devis BellucciValeria Cannillo
Feb 9, 2017·Advanced Healthcare Materials·Piergiorgio GentilePaul V Hatton
Mar 28, 2017·Tissue Engineering. Part a·João S FernandesRui L Reis
Aug 31, 2018·Clinical Oral Investigations·Yusuke TakahashiMikako Hayashi
Jul 25, 2019·Frontiers in Bioengineering and Biotechnology·Saeid KargozarFrancesco Baino
Apr 8, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Mirta JiménezLuis Rojo
Jul 16, 2019·Journal of Materials Science. Materials in Medicine·Breno Rocha BarrioniMarivalda de Magalhães Pereira
Sep 11, 2019·Materials Science & Engineering. C, Materials for Biological Applications·Benjamin KruppkeThomas Hanke
Nov 26, 2019·Materials Science & Engineering. C, Materials for Biological Applications·Romina ShafaghiMark R Towler
Feb 7, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Sajjad FanaeeMohammad-Hossein Nasr Esfahani
Feb 2, 2021·Frontiers in Cell and Developmental Biology·Elia Bosch-RuéRoman A Perez
Jun 3, 2021·International Journal of Molecular Sciences·Li-Ching ChangJen-Tsung Yang
Jun 16, 2018·ACS Applied Materials & Interfaces·Álvaro J LeiteJoão F Mano
Jan 20, 2021·ACS Biomaterials Science & Engineering·Maryam Rahmati, Masoud Mozafari

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Methods Mentioned

BETA
scanning
static light scattering
X‐ray
FCS
scanning electron microscopy

Software Mentioned

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WinX Pow
INCAEnergy
MjoGraph
Coulter LS
Pyris
Excel

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