The outer dynein arm-docking complex: composition and characterization of a subunit (oda1) necessary for outer arm assembly

Molecular Biology of the Cell
Saeko TakadaGeorge B Witman

Abstract

To learn more about how dyneins are targeted to specific sites in the flagellum, we have investigated a factor necessary for binding of outer arm dynein to the axonemal microtubules of Chlamydomonas. This factor, termed the outer dynein arm-docking complex (ODA-DC), previously was shown to be missing from axonemes of the outer dynein armless mutants oda1 and oda3. We have now partially purified the ODA-DC, determined that it contains equimolar amounts of M(r) approximately 105,000 and approximately 70,000 proteins plus a third protein of M(r) approximately 25,000, and found that it is associated with the isolated outer arm in a 1:1 molar ratio. We have cloned a full-length cDNA encoding the M(r) approximately 70,000 protein; the sequence predicts a 62.5-kDa protein with potential homologs in higher ciliated organisms, including humans. Sequencing of corresponding cDNA from strain oda1 revealed it has a mutation resulting in a stop codon just downstream of the initiator ATG; thus, it is unable to make the full-length M(r) approximately 70,000 protein. These results demonstrate that the ODA1 gene encodes the M(r) approximately 70,000 protein, and that the protein is essential for assembly of the ODA-DC and the outer dynein arm on...Continue Reading

References

May 1, 1991·The Journal of Cell Biology·H SakakibaraR Kamiya
May 1, 1991·The Journal of Cell Biology·D R Mitchell, Y Kang
May 24, 1991·Science·A LupasJ Stock
Feb 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·K L Kindle
Jan 1, 1988·Cell Motility and the Cytoskeleton·L T Haimo, R D Fenton
Jan 1, 1986·Methods in Enzymology·S M KingG B Witman
Jan 1, 1986·Methods in Enzymology·G B Witman
Jan 11, 1984·Nucleic Acids Research·J DevereuxO Smithies
Jan 1, 1994·Cell Motility and the Cytoskeleton·C J Brokaw
Aug 1, 1993·The Journal of Cell Biology·H SakakibaraR Kamiya
Jan 1, 1996·Nucleic Acids Research·A BairochK Hofmann
Jan 1, 1996·Methods in Enzymology·A Lupas
Oct 1, 1996·Trends in Biochemical Sciences·A Lupas
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Sep 3, 1998·Molecular Biology of the Cell·M E Fowkes, D R Mitchell
Feb 11, 1999·The Journal of Cell Biology·G J PazourG B Witman
Mar 9, 1999·Molecular Biology of the Cell·M E PorterW Dentler
Mar 21, 2000·Biochimica Et Biophysica Acta·S M King
Apr 4, 2000·Journal of Medical Genetics·M MeeksE Chung
Jan 3, 2001·Methods : a Companion to Methods in Enzymology·G J Pazour, G B Witman

❮ Previous
Next ❯

Citations

Dec 30, 2006·The Journal of Biological Chemistry·Judy FreshourDavid R Mitchell
Apr 6, 2004·Molecular Biology of the Cell·Maureen WirschellGeorge B Witman
Feb 24, 2004·Molecular Biology of the Cell·Haru-aki Yanagisawa, Ritsu Kamiya
Aug 12, 2005·Molecular Biology of the Cell·Noveera T Ahmed, David R Mitchell
Jul 20, 2007·Molecular Biology of the Cell·Miho SakatoStephen M King
Oct 4, 2013·Molecular Biology of the Cell·Anudariya B Dean, David R Mitchell
May 19, 2011·Molecular Human Reproduction·Kazuo Inaba
May 12, 2009·Eukaryotic Cell·Jens BoesgerMaria Mittag
Apr 10, 2003·Eukaryotic Cell·Pushpa KathirCarolyn D Silflow
Jan 15, 2013·International Journal of Plant Genomics·Jens BoesgerMaria Mittag
Mar 8, 2005·American Journal of Respiratory and Critical Care Medicine·Manfred FliegaufHeymut Omran
Oct 13, 2012·Proceedings of the Japan Academy. Series B, Physical and Biological Sciences·Hideo MohriShoji A Baba
Jan 25, 2014·Current Allergy and Asthma Reports·Manuel MataCarmen Carda
Mar 5, 2014·The Journal of Cell Biology·Toshiyuki OdaMasahide Kikkawa
Jun 7, 2006·The Journal of Cell Biology·Ken-ichi Wakabayashi, Stephen M King
Jul 7, 2005·The Journal of Cell Biology·Gregory J PazourGeorge B Witman
Jul 1, 2014·Proceedings of the National Academy of Sciences of the United States of America·Mikito OwaKen-ichi Wakabayashi
Jul 17, 2014·Biophysical Journal·Shohei FujitaMasahide Kikkawa
Feb 20, 2007·The Science of the Total Environment·Karl RopkinsGordon Andrews
Mar 30, 2007·Journal of Molecular Biology·Takashi IshikawaKazuhiro Oiwa
Oct 30, 2003·Cell Motility and the Cytoskeleton·Charles B Lindemann, Alan J Hunt
Jun 23, 2009·Cell Motility and the Cytoskeleton·Ken-Ichi WakabayashiRitsu Kamiya
Mar 17, 2007·Annals of the New York Academy of Sciences·Kazuo Inaba
Jun 24, 2008·Current Biology : CB·Elizabeth C RaffF Rudolf Turner
Oct 2, 2013·Biochimica Et Biophysica Acta·Triscia W HendricksonHenry W Rohrs
Dec 25, 2012·American Journal of Human Genetics·Alexandros OnoufriadisHannah M Mitchison
Dec 25, 2012·American Journal of Human Genetics·Michael R KnowlesUNKNOWN Genetic Disorders of Mucociliary Clearance Consortium
Sep 25, 2016·Cold Spring Harbor Perspectives in Biology·David R Mitchell

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.