PMID: 9182865Jan 1, 1997Paper

The ovarian progestogen receptor in the spotted seatrout, Cynoscion nebulosus, demonstrates steroid specificity different from progesterone receptors in other vertebrates

The Journal of Steroid Biochemistry and Molecular Biology
J Pinter, P Thomas

Abstract

A nuclear progestogen receptor has previously been characterized in the ovary of the spotted seatrout. The steroid specificity of this receptor was further defined in the present study by determining the binding affinity of a wide variety of progestin and corticosteroid agonists and antagonists. The addition of a hydroxyl or keto group to the 11 position resulted in a 10-100-fold decrease in relative binding affinity (RBA). The significance of the 17, 20, and 21 positions in determining the RBA of closely related steroids was investigated in detail. Modification of the 17alpha-hydroxyl to an acetyl or carbyne group resulted in a 10-fold decrease in RBA. The substitution of a ketone group with a hydroxyl group at the 20 position increased binding, whereas the addition of a 21-hydroxyl group consistently decreased RBA by 40-60%. The effect of the 17alpha-hydroxyl group on RBA was dependent on what functional group was present at the 20 position. The addition of a 17alpha-hydroxyl decreased affinity by one- to 10-fold if a ketone group was present at position 20. However, the RBA increased five- to 10-fold upon addition of the 17alpha-hydroxyl group if a hydroxyl was present at the 20 position. The effects of the different substit...Continue Reading

References

Dec 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·B HammondJ McConnell
Aug 1, 1976·Canadian Journal of Physiology and Pharmacology·W A McBlain, V Lewin
Jan 1, 1990·General and Comparative Endocrinology·J S Smith, P Thomas
May 1, 1989·Toxicology and Applied Pharmacology·J WilliamsL Uphouse
Mar 1, 1969·Toxicology and Applied Pharmacology·R M WelchA H Conney
Nov 1, 1981·Journal of Toxicology and Environmental Health·V P Eroschenko
Jun 1, 1994·Human Reproduction·H J KloosterboerW G Schoonen
Oct 1, 1993·Environmental Health Perspectives·T ColbornA M Soto

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Citations

Feb 22, 2011·Molecular and Cellular Endocrinology·Richard N Hanna, Yong Zhu
Sep 3, 2009·Microscopy Research and Technique·Ettore VarricchioMarina Paolucci
Jan 13, 2006·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Anderson O L WongWendy K W Ko
Jan 23, 2015·Environmental Science & Technology·Vimal KumarHana Kocour Kroupová

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