The oxidation of naphthalene and pyrene by cytochrome P450cam

FEBS Letters
P A EnglandL L Wong

Abstract

Mutants of the heme monooxygenase cytochrome P450cam in which Y96 had been replaced with hydrophobic residues, have been shown to oxidise naphthalene and pyrene with rates one to two orders of magnitude faster than the wild-type. Naphthalene was oxidised to 1- and 2-naphthol, probably via the 1,2-oxide intermediate. In the case of the Y96F mutant, naphthalene was oxidised at a rate comparable to camphor. Pyrene oxidation gave 1,6- and 1,8-pyrenequinone with no evidence for attack at the K-region, in contrast to mammalian enzymes. The results show that the Y96 residue plays a key role in controlling the substrate range of P450cam.

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Citations

Apr 22, 2006·Applied Microbiology and Biotechnology·Martin Hofrichter, René Ullrich
Jul 24, 2012·Applied Microbiology and Biotechnology·Stephen G BellLuet-Lok Wong
Jan 27, 2004·Biochemical and Biophysical Research Communications·Nikitas BistolasUlla Wollenberger
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Dec 8, 2009·Bioscience, Biotechnology, and Biochemistry·Toshiki Furuya, Kuniki Kino
Mar 7, 2001·European Journal of Biochemistry·J P JonesL L Wong
Dec 12, 2001·Protein Engineering·S G BellL L Wong
Sep 5, 2017·Chembiochem : a European Journal of Chemical Biology·Md Raihan SarkarStephen G Bell
Mar 7, 2014·Organic & Biomolecular Chemistry·Stephen G BellLuet-Lok Wong
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May 4, 2021·Frontiers in Microbiology·James B Y H Behrendorff
Jul 21, 2006·The Journal of Physical Chemistry. B·Ping YanDavid M Adams

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