The oxidation of p-phenetidine by horseradish peroxidase and prostaglandin synthase and the fate of glutathione during such oxidations.

Biochemical Pharmacology
D RossP Moldéus

Abstract

The oxidation of p-phenetidine by horseradish peroxidase and prostaglandin synthase was investigated. The existence of a free radical intermediate formed during enzymatic oxidation was supported by a ratio of hydrogen peroxide: p-phenetidine consumed of 1:2 in the horseradish peroxidase system. Furthermore in both enzyme systems a rapid oxidation of added glutathione was observed and in the presence of the thiol there was a decreased removal of p-phenetidine. This suggests the reduction of a p-phenetidine radical by glutathione generating p-phenetidine and a thiyl radical. The latter react with oxygen and a rapid oxygen uptake was observed during enzymic oxidation in the presence of thiols. That p-phenetidine radicals were produced during horseradish peroxidase catalyzed oxidation of p-phenetidine was supported by experiments using the spin probe OXANOH. This was oxidized to its stable free radical form (OXANO.) in an enzyme- and substrate-dependent reaction and the EPR signal obtained was not decreased by SOD (80 micrograms/ml) or benzoate (10-100 mM). TLC characteristics of the products of the oxidation of p-phenetidine by both enzymes were almost identical inferring a similar mechanism of oxidation. Two of the metabolites we...Continue Reading

References

May 15, 1978·Biochemical and Biophysical Research Communications·L J MarnettD J Dennison
Aug 11, 1972·Biochimica Et Biophysica Acta·E J Christ, D A van Dorp
Sep 16, 1970·Biochimica Et Biophysica Acta·F Björkstén
Dec 31, 1981·Biochemical and Biophysical Research Communications·B K Sinha
Feb 10, 1983·Biochemical and Biophysical Research Communications·T NohmiM Ishidate
Apr 1, 1982·Biochemical Pharmacology·P MoldéusM Berggren
Jan 9, 2009·Journal of Experimental Botany·Margit BernroitnerChristian Obinger

❮ Previous
Next ❯

Citations

Aug 1, 1989·Biochemical Pharmacology·L ZillettiG P Sgaragli
Jan 1, 1991·Chemico-biological Interactions·B J SmithT E Eling
Jan 1, 1987·Pharmacology & Therapeutics·V V SubrahmanyamP J O'Brien
Jan 1, 1985·Journal of Free Radicals in Biology & Medicine·H WefersH Sies
Feb 1, 1993·Free Radical Biology & Medicine·B E SvenssonP Moldeus
Aug 1, 1991·Pharmacology & Toxicology·T LindqvistB Lindeke
Jun 1, 1988·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·P H Bach, E N Kwizera
Aug 1, 1997·Human & Experimental Toxicology·P HlavicaJ Schulze
Mar 21, 1998·Toxicologic Pathology·J W Verlander
Mar 21, 1998·Toxicologic Pathology·P H Bach, T K Nguyen
Mar 1, 1997·Toxicology and Applied Pharmacology·A P Kulkarni, M P Sajan
Jan 1, 1987·Chemico-biological Interactions·V V SubrahmanyamP J O'Brien
Dec 31, 1985·Chemico-biological Interactions·V V Subrahmanyam, P J O'Brien

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.