The Oxygen Load Supplied during Delivery Room Stabilization of Preterm Infants Modifies the DNA Methylation Profile

The Journal of Pediatrics
Sheila Lorente-PozoMaximo Vento

Abstract

To determine whether the amount of oxygen provided during postnatal stabilization changes the DNA methylome in preterm infants. This prospective, observational study included 32 preterm infants ≤32 weeks of gestation who received oxygen in the delivery room. Patients were monitored using a respiratory function monitor to determine the amount of oxygen received upon stabilization. Blood samples were processed for comparison of DNA methylation before and after resuscitation using a DNA methylation high-resolution microarray Infinium Human DNA methylation EPIC 850K BeadChip. The median amount oxygen provided to preterm infants during stabilization was 644 mLO2/kg. Male sex and vaginal delivery were associated with increased oxygen needs. There were 2626 differentially methylated CpGs representing 1567 genes that showed an association with oxygen load selected and, of these, 85% were hypomethylated. We found that oxygen loads of >500 mLO2/kg changed the methylation pattern of the selected CpGs. Genes associated with these CpGs were "enriched" in KEGG pathways involved in cell cycle progression, DNA repair, and oxidative stress. The oxygen load provided upon resuscitation modified the DNA methylome. Differential methylation may lead...Continue Reading

Citations

Feb 23, 2019·Children·Inmaculada Lara-CantónMáximo Vento
Aug 4, 2020·Journal of Developmental Origins of Health and Disease·Vitor TeixeiraJean-Claude Lavoie
Oct 10, 2018·Pediatric Research·Ola Didrik SaugstadMaximo Vento
Jan 30, 2021·Seminars in Fetal & Neonatal Medicine·Praveen ChandrasekharanSteven H Abman
Dec 11, 2020·International Journal of Molecular Sciences·Yves MenezoKay Elder

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