PMID: 12752444May 20, 2003Paper

The oxygenase component of phenol hydroxylase from Acinetobacter radioresistens S13

European Journal of Biochemistry
Sara DivariCarlo Giunta

Abstract

Phenol hydroxylase (PH) from Acinetobacter radioresistens S13 represents an example of multicomponent aromatic ring monooxygenase made up of three moieties: a reductase (PHR), an oxygenase (PHO) and a regulative component (PHI). The function of the oxygenase component (PHO), here characterized for the first time, is to bind molecular oxygen and catalyse the mono-hydroxylation of substrates (phenol, and with less efficiency, chloro- and methyl-phenol and naphthol). PHO was purified from extracts of A. radioresistens S13 cells and shown to be a dimer of 206 kDa. Each monomer is composed by three subunits: alpha (54 kDa), beta (38 kDa) and gamma (11 kDa). The gene encoding PHO alpha (named mopN) was cloned and sequenced and the corresponding amino acid sequence matched with that of functionally related oxygenases. By structural alignment with the catalytic subunits of methane monooxygenase (MMO) and alkene monooxygenase, we propose that PHO alpha contains the enzyme active site, harbouring a dinuclear iron centre Fe-O-Fe, as also suggested by spectral analysis. Conserved hydrophobic amino acids known to define the substrate recognition pocket, are also present in the alpha-subunit. The prevalence of alpha-helices (99.6%) as studie...Continue Reading

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Citations

Apr 7, 2004·Applied and Environmental Microbiology·Valeria CafaroAlberto Di Donato
Feb 4, 2005·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Giovanna Di NardoCarlo Giunta
Aug 25, 2015·Water, Air, and Soil Pollution·Dorota DomaradzkaDanuta Wojcieszyńska
Jul 16, 2014·Journal of Environmental Management·Danuta WojcieszyńskaUrszula Guzik
Apr 9, 2013·Bioresource Technology·Cintia C LoboEdgardo M Contreras
Mar 21, 2009·Chembiochem : a European Journal of Chemical Biology·Raffaella CaglioCarlo Giunta
Nov 16, 2019·World Journal of Microbiology & Biotechnology·B M Alanis-SánchezJ Jan-Roblero
Sep 5, 2020·Drug Design, Development and Therapy·Xolani H MakhobaOfentse J Pooe

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