The p110 isoform of the CDP/Cux transcription factor accelerates entry into S phase

Molecular and Cellular Biology
Laurent SansregretAlain Nepveu

Abstract

The CDP/Cux transcription factor was previously found to acquire distinct DNA binding and transcriptional properties following a proteolytic processing event that takes place at the G1/S transition of the cell cycle. In the present study, we have investigated the role of the CDP/Cux processed isoform, p110, in cell cycle progression. Populations of cells stably expressing p110 CDP/Cux displayed a faster division rate and reached higher saturation density than control cells carrying the empty vector. p110 CDP/Cux cells reached the next S phase faster than control cells under various experimental conditions: following cell synchronization in G0 by growth factor deprivation, synchronization in S phase by double thymidine block treatment, or enrichment in G2 by centrifugal elutriation. In each case, duration of the G1 phase was shortened by 2 to 4 h. Gene inactivation confirmed the role of CDP/Cux as an accelerator of cell cycle progression, since mouse embryo fibroblasts obtained from Cutl1z/z mutant mice displayed a longer G1 phase and proliferated more slowly than their wild-type counterparts. The delay to enter S phase persisted following immortalization by the 3T3 protocol and transformation with H-RasV12. Moreover, CDP/Cux in...Continue Reading

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Citations

Jan 16, 2010·The Journal of Biological Chemistry·Slavko ČeruNataša Kopitar-Jerala
Aug 24, 2010·The Journal of Biological Chemistry·Laurent SansregretAlain Nepveu
Apr 19, 2012·Preparative Biochemistry & Biotechnology·Jirka GrosseDaniela Grimm
Feb 10, 2012·Nucleic Acids Research·Charles VadnaisAlain Nepveu
Nov 19, 2008·Molecular and Cellular Biology·Laetitia SeguinJacques Bertoglio
Feb 8, 2008·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·George StratigopoulosRudolph L Leibel
Feb 26, 2010·Cell Division·C Elizabeth Caldon, Elizabeth A Musgrove
Jan 17, 2013·Breast Cancer Research : BCR·Kelly K HuntKhandan Keyomarsi
Mar 13, 2014·PLoS Biology·Zubaidah M RamdzanAlain Nepveu
Jun 2, 2009·Frontiers in Bioscience (Landmark Edition)·Neal I Alcalay, Gregory B Vanden Heuvel
Sep 6, 2014·Nature Reviews. Cancer·Zubaidah M Ramdzan, Alain Nepveu
Mar 4, 2008·Gene·Laurent Sansregret, Alain Nepveu
Apr 6, 2011·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Binu M PaulGregory B Vanden Heuvel
Jul 1, 2010·Birth Defects Research. Part A, Clinical and Molecular Teratology·Partha MukhopadhyayRobert M Greene
May 9, 2015·The Journal of Surgical Research·Daniela StangelJörg Kleeff
Jul 25, 2012·Cell Cycle·Muriel DavidJacques Bertoglio
Jan 20, 2011·Proceedings of the National Academy of Sciences of the United States of America·Laurent SansregretAlain Nepveu
Feb 2, 2017·Oncotarget·Zubaidah M RamdzanAlain Nepveu
Oct 28, 2008·The Journal of Biological Chemistry·Brian J WilsonAlain Nepveu
Apr 22, 2018·Nature Reviews. Cardiology·Cong-Lin LiuGuo-Ping Shi
Aug 5, 2019·Journal of Molecular Neuroscience : MN·Xiujie WuXueyuan Heng
Mar 22, 2008·The Journal of Biological Chemistry·Chantal CadieuxMaxime Bouchard
Mar 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Soji SebastianJyotsna Dhawan
Jun 18, 2020·Frontiers in Oncology·Ning LiuHailong Wu
Apr 12, 2008·Molecular Cancer Research : MCR·Brigitte GouletAlain Nepveu

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