The P1N-PISPO trans-Frame Gene of Sweet Potato Feathery Mottle Potyvirus Is Produced during Virus Infection and Functions as an RNA Silencing Suppressor

Journal of Virology
Ares MingotJuan José López-Moya

Abstract

The positive-sense RNA genome of Sweet potato feathery mottle virus (SPFMV) (genus Potyvirus, family Potyviridae) contains a large open reading frame (ORF) of 3,494 codons translatable as a polyprotein and two embedded shorter ORFs in the -1 frame: PISPO, of 230 codons, and PIPO, of 66 codons, located in the P1 and P3 regions, respectively. PISPO is specific to some sweet potato-infecting potyviruses, while PIPO is present in all potyvirids. In SPFMV these two extra ORFs are preceded by conserved G2A6 motifs. We have shown recently that a polymerase slippage mechanism at these sites could produce transcripts bringing these ORFs in frame with the upstream polyprotein, thus leading to P1N-PISPO and P3N-PIPO products (B. Rodamilans, A. Valli, A. Mingot, D. San Leon, D. B. Baulcombe, J. J. Lopez-Moya, and J.A. Garcia, J Virol 89:6965-6967, 2015, doi:10.1128/JVI.00337-15). Here, we demonstrate by liquid chromatography coupled to mass spectrometry that both P1 and P1N-PISPO are produced during viral infection and coexist in SPFMV-infected Ipomoea batatas plants. Interestingly, transient expression of SPFMV gene products coagroinfiltrated with a reporter gene in Nicotiana benthamiana revealed that P1N-PISPO acts as an RNA silencing su...Continue Reading

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Citations

Jun 4, 2016·Genome Announcements·Solomon MainaRoger A C Jones
Dec 21, 2016·Molecular Plant Pathology·Mingjun LiYuqin Cheng
Apr 6, 2019·Critical Reviews in Biotechnology·Ashish PrasadManoj Prasad
Jul 13, 2019·Annual Review of Virology·Hongguang Cui, Aiming Wang
Sep 11, 2019·Phytopathology·Maria Luisa Domingo-CalapJuan José López-Moya
Jan 26, 2020·Viruses·Adrian J GibbsRoger A C Jones
Feb 16, 2018·Journal of Virology·Araiz GalloJuan Antonio García
Sep 24, 2019·Phytopathology·Ana Beatriz Moreno, Juan José López-Moya
Jun 8, 2017·Annual Review of Phytopathology·Ikkei ShikanoGary W Felton
Apr 18, 2020·Frontiers in Plant Science·Jan F KreuzeWilmer J Cuellar
Apr 24, 2021·Annual Review of Phytopathology·Xiuling YangAiming Wang

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