The P2X(7) receptor from Xenopus laevis: formation of a large pore in Xenopus oocytes

FEBS Letters
Martin PaukertStefan Gründer

Abstract

The purinergic P2X(7) receptor is an ATP-receptor channel predominantly expressed in immune cells. P2X(7) has been cloned from human, rat and mouse. Here we report cloning of the Xenopus laevis P2X(7) receptor (xP2X(7)). xP2X(7) is only about 50% identical to the mammalian homologues, shows a broad tissue expression pattern, and has the electrophysiological characteristics typical of a P2X(7) receptor: low agonist affinity (EC(50) about 2.6 mM) and a non-desensitizing current. Moreover, expression of xP2X(7) in Xenopus oocytes is sufficient to induce the formation of a large pore, which is permeable to large cations such as NMDG(+). Identification of a non-mammalian P2X(7) receptor may help to identify functionally important parts of the protein.

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Citations

May 2, 2012·Purinergic Signalling·Karina Kaczmarek-HájekAnnette Nicke
Aug 12, 2008·Proceedings of the National Academy of Sciences of the United States of America·Severine Chaumont, Baljit S Khakh
Jan 22, 2005·Clinical and Experimental Pharmacology & Physiology·M MonifD A Williams
Sep 30, 2010·Cancer Cell International·Héctor E López-ValdésAndrew C Charles
Mar 9, 2010·The International Journal of Developmental Biology·Timothy J Hinterberger
Apr 5, 2011·British Journal of Pharmacology·Helen J BradleyLin-Hua Jiang
Jan 17, 2015·Pflügers Archiv : European journal of physiology·Michaela StolzFritz Markwardt
Feb 6, 2015·Nature Communications·Jiraporn OusingsawatKarl Kunzelmann
Aug 12, 2005·American Journal of Physiology. Cell Physiology·Lin-Hua JiangR Alan North
Sep 1, 2007·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Ronald SluyterJames S Wiley
Sep 25, 2002·Physiological Reviews·R Alan North
Aug 14, 2019·Frontiers in Cellular Neuroscience·Camille BlanchardKarine Massé
Feb 3, 2007·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Monika Sundqvist
Feb 4, 2015·The Journal of Biological Chemistry·Xin LiangTerrance M Egan
Aug 5, 2004·The Journal of Biological Chemistry·Kelvin C AgbohSteven J Ennion
Nov 14, 2020·International Journal of Molecular Sciences·María Ángeles Martínez-CuestaÁngeles Álvarez

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