The P2Y₄ receptor forms homo-oligomeric complexes in several CNS and PNS neuronal cells.

Purinergic Signalling
Nadia D'AmbrosiCinzia Volonté

Abstract

It is well established that several cell surface receptors interact with each other to form dimers and oligomers, which are essential for their activation. Since little is known about the quaternary structure of P2Y receptors, in the present work, we investigated the expression of the G-protein-coupled P2Y₄ subunit as monomeric or higher-order complex protein. We examined both endogenously expressed P2Y₄ subtype with the aid of specific anti-P2Y₄ antiserum, and heterologously transfected P2Y₄-tagged receptors with the use of antitag antibodies. In both cases, we found the P2Y₄ receptor displaying molecular masses corresponding to monomeric, dimeric and oligomeric structures. Experiments performed in the absence of reducing agents demonstrated that there is a strict correlation among the multiple protein bands and that the multimeric forms are at least partially assembled by disulphide bonds. The direct demonstration of P2Y₄ homodimerisation comes instead from co-transfection and differential co-immunoprecipitation experiments, with the use of differently tagged P2Y₄ receptors and antitag antibodies. The structural propensity of the P2Y₄ protein to form homo-oligomers may open the possibility of a novel regulatory mechanism of p...Continue Reading

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Citations

Feb 15, 2008·Naunyn-Schmiedeberg's Archives of Pharmacology·Laszlo KölesPeter Illes
Jun 28, 2011·Pharmacology & Therapeutics·Cinzia VolontéNadia D'Ambrosi
May 9, 2009·British Journal of Pharmacology·Amarnath TalasilaJohn M Dickenson
May 8, 2007·Biochimica Et Biophysica Acta·Nadia D'AmbrosiCinzia Volonté
Jun 27, 2017·Pharmacology & Therapeutics·Akiyuki NishimuraMotohiro Nishida
Feb 11, 2020·International Reviews of Immunology·Marharyta Zyma, Rafał Pawliczak
Jul 29, 2017·Frontiers in Pharmacology·Andromeda Liñán-RicoFievos L Christofi
Aug 1, 2020·Frontiers in Immunology·Davide FerrariMarco Idzko

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Methods Mentioned

BETA
FCS
immunoprecipitation
electrophoresis
imaging technique
transfection
glycosylation

Software Mentioned

Kodak

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