The P450-1 gene of Gibberella fujikuroi encodes a multifunctional enzyme in gibberellin biosynthesis

Proceedings of the National Academy of Sciences of the United States of America
M Cecilia RojasBettina Tudzynski

Abstract

Recent studies have shown that the genes of the gibberellin (GA) biosynthesis pathway in the fungus Gibberella fujikuroi are organized in a cluster of at least seven genes. P450-1 is one of four cytochrome P450 monooxygenase genes in this cluster. Disruption of the P450-1 gene in the GA-producing wild-type strain IMI 58289 led to total loss of GA production. Analysis of the P450-1-disrupted mutants indicated that GA biosynthesis was blocked immediately after ent-kaurenoic acid. The function of the P450-1 gene product was investigated further by inserting the gene into mutants of G. fujikuroi that lack the entire GA gene cluster; the gene was highly expressed under GA production conditions in the absence of the other GA-biosynthesis genes. Cultures of transformants containing P450-1 converted ent-[(14)C]kaurenoic acid efficiently into [(14)C]GA(14), indicating that P450-1 catalyzes four sequential steps in the GA-biosynthetic pathway: 7beta-hydroxylation, contraction of ring B by oxidation at C-6, 3beta-hydroxylation, and oxidation at C-7. The GA precursors ent-7alpha-hydroxy[(14)C]kaurenoic acid, [(14)C]GA(12)-aldehyde, and [(14)C]GA(12) were also converted to [(14)C]GA(14). In addition, there is an indication that P450-1 may a...Continue Reading

References

Nov 9, 1990·Biochimica Et Biophysica Acta·P J AshmanP M Bramley
Apr 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·G M Church, W Gilbert
Mar 1, 1995·The Journal of Steroid Biochemistry and Molecular Biology·T SunM Okamoto
Jun 23, 1995·The Journal of Biological Chemistry·R Fernández-MartínE Cerdá-Olmedo
Dec 1, 1994·Plant Molecular Biology·R Hooley
Jun 10, 1997·Proceedings of the National Academy of Sciences of the United States of America·T Lange
Jan 26, 1999·Fungal Genetics and Biology : FG & B·B Tudzynski, K Hölter
Dec 20, 2000·Trends in Plant Science·P Hedden, A L Phillips
Dec 29, 2000·Nature·UNKNOWN Arabidopsis Genome Initiative
Feb 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·C A HelliwellW J Peacock

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Citations

Dec 4, 2004·Applied Microbiology and Biotechnology·Bettina Tudzynski
Jun 1, 2011·World Journal of Microbiology & Biotechnology·Erika Y Rios-IribeEleazar M Escamilla-Silva
Nov 1, 2002·Fungal Genetics and Biology : FG & B·Pia LinnemannstönsBettina Tudzynski
Feb 2, 2011·Journal of the American Chemical Society·Georg ZocherChristian Hertweck
Jul 24, 2012·Natural Product Reports·Larissa M Podust, David H Sherman
Sep 5, 2002·The Plant Journal : for Cell and Molecular Biology·Yuji SawadaShin-Ichi Ayabe
May 29, 2008·Proceedings of the National Academy of Sciences of the United States of America·Jing-Ke WengClint Chapple
Jan 17, 2003·Plant Physiology·Sandra E DavidsonJames B Reid
Jun 24, 2008·Applied and Environmental Microbiology·Christiane BömkeBettina Tudzynski
Apr 12, 2012·Applied and Environmental Microbiology·Lena StudtBettina Tudzynski
Jul 27, 2001·Applied and Environmental Microbiology·B TudzynskiP Gaskin
Apr 7, 2004·Applied and Environmental Microbiology·S P McCormickA E Desjardins
Jan 5, 2008·Annual Review of Plant Biology·Shinjiro Yamaguchi
Jan 28, 2004·Bioscience, Biotechnology, and Biochemistry·Tomonobu ToyomasuHideaki Oikawa
Jan 1, 2014·Phytochemistry·Constanza MéndezMaría Cecilia Rojas
Jan 1, 2002·The Arabidopsis Book·Danièle Werck-ReichhartSuzanne Paquette
Jul 14, 2010·Biochimica Et Biophysica Acta·B Crešnar, S Petrič
Jul 29, 2008·Fungal Genetics and Biology : FG & B·Martin J SpieringChristopher L Schardl
Feb 12, 2008·Mycological Research·Sanjay SaikiaBarry Scott
Nov 3, 2015·Journal of Plant Growth Regulation·Peter Hedden, Valerie Sponsel
Jun 13, 2006·Fungal Genetics and Biology : FG & B·Carolyn A YoungBarry Scott
Apr 27, 2012·The Biochemical Journal·Peter Hedden, Stephen G Thomas
Aug 13, 2008·Fungal Genetics and Biology : FG & B·Elena A TsavkelovaBettina Tudzynski
Nov 26, 2014·Functional & Integrative Genomics·Agnieszka A GoliczJacqueline Batley
Jun 12, 2013·FEMS Microbiology Letters·Ron CaspiPeter D Karp
Nov 15, 2016·Nature Chemical Biology·Ryan S NettReuben J Peters

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