The P450 monooxygenase BcABA1 is essential for abscisic acid biosynthesis in Botrytis cinerea

Applied and Environmental Microbiology
Verena SiewersPaul Tudzynski

Abstract

The phytopathogenic ascomycete Botrytis cinerea is known to produce abscisic acid (ABA), which is thought to be involved in host-pathogen interaction. Biochemical analyses had previously shown that, in contrast to higher plants, the fungal ABA biosynthesis probably does not proceed via carotenoids but involves direct cyclization of farnesyl diphosphate and subsequent oxidation steps. We present here evidence that this "direct" pathway is indeed the only one used by an ABA-overproducing strain of B. cinerea. Targeted inactivation of the gene bccpr1 encoding a cytochrome P450 oxidoreductase reduced the ABA production significantly, proving the involvement of P450 monooxygenases in the pathway. Expression analysis of 28 different putative P450 monooxygenase genes revealed two that were induced under ABA biosynthesis conditions. Targeted inactivation showed that one of these, bcaba1, is essential for ABA biosynthesis: DeltaBcaba1 mutants contained no residual ABA. Thus, bcaba1 represents the first identified fungal ABA biosynthetic gene.

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Citations

Mar 19, 2011·Journal of Plant Research·Daisuke TakezawaYoichi Sakata
Jan 13, 2006·Molecular Plant-microbe Interactions : MPMI·Véronique ChaguéAmir Sharon
May 9, 2009·Science·Murray R Grant, Jonathan D G Jones
Jul 6, 2006·Applied and Environmental Microbiology·Verena SiewersPaul Tudzynski
May 3, 2005·Annual Review of Plant Biology·Eiji Nambara, Annie Marion-Poll
Jul 1, 2008·Current Opinion in Plant Biology·Miguel Angel LópezCarmen Castresana
Mar 2, 2016·Biochemical and Biophysical Research Communications·Carla C UrangaRufina Hernández-Martínez
Nov 21, 2007·Fungal Genetics and Biology : FG & B·Ljerka LahRadovan Komel
Jul 1, 2015·Current Opinion in Plant Biology·Carla Spence, Harsh Bais
Jan 28, 2015·Molecular Plant-microbe Interactions : MPMI·Julia SchumacherBettina Tudzynski
Nov 8, 2007·FEMS Microbiology Letters·Mathias ChoquerMuriel Viaud
Jul 28, 2016·Bioengineered·Tian-Qiong ShiXiao-Jun Ji

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