PMID: 3054805Aug 1, 1988Paper

The packing of acetylcholine into quanta at the frog neuromuscular junction is inhibited by increases in intracellular sodium

Pflügers Archiv : European journal of physiology
W Van der Kloot

Abstract

Pretreatment with hypertonic solutions, insulin, or adrenaline increases the size of quanta at the frog neuromuscular junction, as determined by measurements of miniature end plate potentials or currents (Van der Kloot and Van der Kloot 1985, 1986). The increase in quantal size apparently is due to an increase in acetylcholine (ACh) content of individual quanta. These treatments, therefore, can be used to study the packaging of ACh. Previously, I reported that increases are blocked by an inhibitor of active ACh uptake into vesicles (Van der Kloot 1986b, 1987b). The present study shows that the increases in quantal size were antagonized by inhibiting the Na+-K+ exchange pump with 100 microM ouabain, 10 microM dihydroouabain, or K+-free solutions. The increases in quantal size were also antagonized by 10 microM monensin, a Na+ ionophore, or by 5 microM aconitine, which opens Na+ channels at normal resting potentials. Apparently a rise in intracellular [Na+] inhibits the addition of ACh to quanta. The mechanism by which a rise in intracellular Na+ inhibits ACh packing is unknown, but apparently it is not due to inhibition of choline reuptake into the terminals. Also consistent with the above hypothesis is that the increase in quan...Continue Reading

References

Jul 1, 1975·British Journal of Pharmacology·I G Marshall, R L Parsons
Mar 16, 1979·Pflügers Archiv : European journal of physiology·D AttwellC Ojeda
Jan 1, 1986·Pflügers Archiv : European journal of physiology·W Van der Kloot
Jun 25, 1986·Brain Research·W van der Kloot, T E van der Kloot
Jul 1, 1986·Physiological Reviews·T Clausen
Oct 1, 1987·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·W Van der Kloot
Jan 15, 1985·Experientia·W Van der Kloot, T E Van der Kloot
May 1, 1970·British Journal of Pharmacology·I G Marshall
Jun 11, 1974·Pflügers Archiv : European journal of physiology·H Schmidt, O Schmitt
Nov 10, 1972·Science·H I Yamamura, S H Snyder
Apr 1, 1983·Pflügers Archiv : European journal of physiology·W Van der Kloot, R Latta
Oct 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·W P Melega, B D Howard
Apr 1, 1965·The Journal of Physiology·D ELMQVIST, D M QUASTEL

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Citations

Jan 1, 1991·Progress in Neurobiology·W Van der Kloot
Sep 1, 1990·The British Journal of Surgery·E J van Rhede van der Kloot, P J Jörning
Mar 16, 2000·Reviews in the Neurosciences·D Sulzer, E N Pothos

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