The PapilloCheck Assay for Detection of High-Grade Cervical Intraepithelial Neoplasia

Journal of Clinical Microbiology
Emma J CrosbieHenry C Kitchener

Abstract

Human papillomavirus (HPV) testing is used in primary cervical screening, as an adjunct to cervical cytology for the management of low grade abnormal cytology, and in a test of cure. PapilloCheck (Greiner Bio-One) is a PCR-based DNA microarray system that can individually identify 24 HPV types, including the 13 high-risk (HR) types identified by Hybrid Capture 2 (HC2). Here, we compare PapilloCheck with HC2 for the detection of high-grade cervical intraepithelial neoplasia (CIN2+) in a total of 8,610 cervical cytology samples from the ARTISTIC population-based cervical screening study. We performed a retrospective analysis of 3,518 cytology samples from round 1 ARTISTIC enriched for underlying CIN2+ (n = 723) and a prospective analysis of 5,092 samples from round 3 ARTISTIC. Discrepant results were tested using the Roche reverse line blot (RLB) or Linear Array (LA) assay. The relative sensitivity and specificity of HR PapilloCheck compared with that of HC2 for the detection of CIN2+ in women aged over 30 years were 0.94 (95% confidence interval [CI], 0.91, 0.97) and 1.05 (95% CI, 1.04, 1.05), respectively. HC2 missed 44/672 (7%) CIN2+ lesions, while HR PapilloCheck missed 74/672 (11%) CIN2+ lesions. Thirty-six percent of HC2-po...Continue Reading

References

Aug 19, 1999·The Journal of Pathology·J M WalboomersN Muñoz
May 4, 2000·Journal of the National Cancer Institute·H zur Hausen
Aug 16, 2001·Journal of Medical Virology·G TerryA Lorincz
Mar 29, 2002·Journal of Clinical Pathology·F X BoschK V Shah
Jun 9, 2004·Journal of Clinical Microbiology·S-M KulmalaK Syrjänen
Nov 1, 2008·International Journal of Cancer. Journal International Du Cancer·Chris J L M MeijerPeter J F Snijders
Dec 17, 2008·European Journal of Cancer Prevention : the Official Journal of the European Cancer Prevention Organisation (ECP)·Jin-Kyoung OhHai-Rim Shin
Apr 8, 2009·The Lancet Oncology·Véronique BouvardUNKNOWN WHO International Agency for Research on Cancer Monograph Working Group
Apr 28, 2009·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·J JonesS Hibbitts
Nov 7, 2009·Health Technology Assessment : HTA·H C KitchenerUNKNOWN ARTISTIC Trial Study Group
Nov 27, 2009·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Philippe HalfonMaria Teresa Sandri
Feb 14, 2013·Cytopathology : Official Journal of the British Society for Clinical Cytology·N GhosalS P Kumaran
Apr 27, 2013·Lancet·Emma J CrosbieHenry C Kitchener
Aug 2, 2013·Cytopathology : Official Journal of the British Society for Clinical Cytology·M VijayvargiyaV K Iyer
Aug 9, 2013·BMJ : British Medical Journal·Henry C KitchenerEmma J Crosbie

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Citations

Jun 5, 2016·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·I HeardM Arbyn
Jun 21, 2019·Health Technology Assessment : HTA·Clare GilhamJulian Peto
Feb 7, 2017·Expert Reviews in Molecular Medicine·D TsakogiannisP Markoulatos
Nov 17, 2019·European Annals of Otorhinolaryngology, Head and Neck Diseases·A HolmK Olofsson

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